Cell Biology Assays
Essential Methods
Published on 19. November 2009
298 pages
978-0-12-375153-9 (ISBN)
Description
This text provides comprehensive protocols essential methods across cell biology. The techniques in this text are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls while enabling researchers at all stages to embark on basic problems using a variety of technologies and model systems.
- Provides researchers with solutions in lab environments
- Features an array of essential methods, including endocytic pathways, membranes, mitochondria, and in vitro motility
- Information on a plethora of technologies needed to tackle complex problems
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11/2009
Academic Press
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Content
- Permeabilized Epithelial Cells to Study Exocytic Membrane Transport
- Studying Excit and Surface Delivery of Post-Golgi Transport Intermediates Using in vitro and Live-Cell Microscopy-Based Approaches
- Assays Measuring Membrane Transport in the Endocytic Pathway
- Microsome-Based Assay for Analysis of Endoplasmic Reticulum to Golgi Transport In Mammalian Cells
- Cotranslational Translocation of Proteins into Microsomes Derived from the Rough Endoplasmic Reticulum of Mammalian Cells
- Use of Permeabilsed Mast Cells to Analyze Regulated Exocytosis
- Syringe Loading: A Method for Assessing Plasma Membrane Function as a Reflection of Mechanically Induced Cell Loading
- Cell Surface Biotinylation and Other Techniques for Determination of Surface Polarity of Epithelial Monolayers
- Protein Translocation into Mitochondria
- Polarographic Assays of Mitochrondrial Functions
- Analysis of Nuclear Protein Import and Export in Digitonin-Permeabilized Cells
- Heterokaryons: An Assay for Nucleocytoplasmic Shuttling
- DNA Replication-Dependent Chromatin Assembly System
- Cygnets: Intracellular Guanosine 3',5-Cycli Monophosphate Sensing in Primary Cells Using Fluorescence Energy Transfer
- Ca2+ as a Second Messenger: New Reporters for Calcium (Cameleons and Camgaroos)
- Ratiometric Pericam
- Fuorescent Indicators for Imaging Protein Phosphorylation in Single Living Cells
- In Situ Electroporation of Radioactive Nucleotides: Assessment of Ras Activity or 32P Labeling of Cellular Proteins
- Dissecting Pathways; in Situ Electroporation for the Study of Signal Transduction and Gap Junctional Communication
- Detection of Protein-Protein Interactions in vivo Using Cyan and Yellow Fluorescent Proteins
- Tracking Individual Chromosomes with Integrated Arrays of Iacop Sites and GFP-laci Repressor: Analyzing Position and Dynamics of Chromosomal Loci in Saccharomyces cerevisiae
- Microtubule Motility Assays
- In vitro Assays for Mitotic Spindle Assembly and Function
- In vitro Motility Assays with Actin
- Use of Brain Cytosolic Extracts for Studying Actin-Based Motility of Listeria monocytogenes
- Pedestal Formation by Pathogenic Escherichia coli: A Model System for Studying Signal Transduction to the Actin Cytoskeleton
- Listeria monocytogenes: Techniques to Analyze Bacterial Infection in vitro
- Measurement of Cellular Contractile Forces Using Patterned Elastomer
