PCR Protocols
A Guide to Methods and Applications
Published on 2. December 2012
482 pages
978-0-08-088671-8 (ISBN)
Description
The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual.
- Avoid contamination--with specific instructions on setting up your lab
- Avoid cumbersome molecular biological techniques
- Discover new applications
More details
Other editions
Additional editions
Michael A. Innis | David H. Gelfand | John J. Sninsky
PCR Protocols
A Guide to Methods and Applications
Book
01/1990
Academic Press
€54.46
Withdrawn from sale
Content
[Contents in a condensed version]Basic MethodologyAmplification of genomic DNA targetsOptimization of PCR reaction and design of primersNonradioactive detection of PCR productsmRNA quantitation by PCRCloning PCR productsContamination and product carry-overPCR from paraffin sectionsAmplifying ancient DNAResearch ApplicationsDirect DNA sequencing of PCR productsDNA footprintingDetection of rare mRNAsSite-directed mutagenesisScreening DNA librariesAmplification of flanking DNA sequencesGenetics and EvoltionTissue typing and disease susceptibilityHaplotype analysis of single cellsPopulation biologyMultiplex DNA amplificationDiagnostics and ForensicsHIV detectionHTLV I and II detectionScreening for novel virusesDetection of oncogenic mutationsDiagnosis of retinoblastomaIndividual identificationInstruments and SuppliesOrganizing a laboratory for PCR workLow-cost air-driven cycling ovenBasic equipment and supplies for the PCR lab
