The Immunological Synapse Part A
Academic Press
Published on 16. January 2023
206 pages
978-0-323-90155-0 (ISBN)
Description
The Immunological Synapse, Part A, Volume 173 in the Methods in Cell Biology series provides state-of-the-art methods for the study of the immunological synapse. Sections cover Imaging polarized granule release at the cytotoxic T cell immunological synapse using TIRF microscopy: control by polarity regulators, Analysis of centrosomal area actin reorganization and centrosome polarization upon lymphocyte activation at the immunological synapse, P815-based redirected degranulation assay to study human NK cell effector functions, Cytotoxic and Chemotactic Dynamics of Natural Killer Cells Quantified by Live-cell Imaging, Quantification of interaction frequency between antigen-presenting cells and T cells by conjugation assay, and more.
Other chapters focus on the Study of the Effects of NK-Tumor Cell Interaction by Proteomic Analysis and Imaging, Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers, Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers, An in vitro model to monitor natural killer cell effector functions against primary breast cancer, and Standardized Protocol for the Evaluation of Chimeric Antigen Receptor (CAR)-modified Cell Immunological Synapse Quality using the Glass-supported Planar L.
- Covers various methods related to the study of the immunological synapse
- Includes detailed, point-by-point, methods as well as various important notes
- Provides the authority and expertise from an international board of leading scientists
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Clement Thomas | Lorenzo Galluzzi
The Immunological Synapse Part A: Volume 173
Book
01/2023
Academic Press
€172.08
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Persons
Content
- Imaging polarized granule release at the cytotoxic T cell immunological synapse using TIRF microscopy: Control by polarity regulatorsMarie Juzans, Celine Cuche, Vincenzo Di Bartolo, and Andres Alcover
- Analysis of centrosomal area actin reorganization and centrosome polarization upon lymphocyte activation at the immunological synapseSara Fernandez-Hermira, Irene Sanz-Fernandez, Marta Botas, Victor Calvo, and Manuel Izquierdo
- P815-based redirected degranulation assay to study human NK cell effector functionsIñigo Terren, Gabirel Astarloa-Pando, Ainhoa Amarilla-Irusta, and Francisco Borrego
- Cytotoxic and chemotactic dynamics of NK cells quantified by live-cell imagingYanting Zhu and Jue Shi
- Quantification of interaction frequency between antigen-presenting cells and T cells by conjugation assayOndrej Cerny
- Assessment of membrane lipid state at the natural killer cell immunological synapseYu Li and Jordan S. Orange
- Study of the effects of NK-tumor cell interaction by proteomic analysis and imagingChiara Lavarello, Paola Orecchia, Andrea Petretto, Massimo Vitale, Claudia Cantoni, and Monica Parodi
- Protocol for the murine antibody-dependent cellular phagocytosis assayEliana Stanganello, Magdalena Brkic, Steven Zenner, Ines Beulshausen, Ute Schmitt, and Fulvia Vascotto
- Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titersMelanie Grusdat, Catherine Dostert, and Dirk Brenner
- An in vitro model to monitor natural killer cell effector functions against breast cancer cells derived from human tumor tissueNicky A. Beelen, Femke A.I. Ehlers, Loes F.S. Kooreman, Gerard M.J. Bos, and Lotte Wieten
- Standardized protocol for the evaluation of chimeric antigen receptor (CAR)-modified cell immunological synapse quality using the glass-supported planar lipid bilayerJong Hyun Cho, Wei-chung Tsao, Alireza Naghizadeh, and Dongfang Liu
- Potency monitoring of CAR T cellsDongrui Wang, Xin Yang, Agata Xella, Lawrence A. Stern, and Christine E. Brown
