Immunochemistry LabFax

¿ContributorsAbbreviations1. Plasma Proteins Plasma and Serum Anticoagulants Properties of Commonly Used Anticoagulants (Table 1) Serum Preparation of Serum from Plasma (Reversal of Anticoagulants) (Table 2) Plasma/Serum Proteins Human Blood Plasma Proteins in Binding and Transport of Ligands (Table 3) Human Blood Plasma Apolipoproteins (Table 4) Human Blood Plasma Proteins in Coagulation and Fibrinolysis (Table 5) Human Blood Plasma Proteinase Inhibitors (Table 6) Human Immunoglobulins (Table 7) Human Complement Components (Table 8) Factors Affecting the Concentration of Plasma Proteins The Effect of Age Protein, Albumin, ¿-Globulin Plasma Concentrations (Table 9) Concentration of Immunoglobulin Isotypes in Plasma (Table 10) Concentration of IgG Subclasses in Plasma (Table 11) Concentration of C3, C4 and C5 in Plasma (Table 12) The Acute Phase Response Acute Phase Proteins (Table 13) Plasma Proteins in Other Body Fluids Concentrations of Immunoglobulins and Albumin in Body Fluids (Table 14) Antibodies Recognizing Plasma/Serum Proteins Absorption of Unwanted Specificities Anti-Whole Sera Antibodies Purified Antibodies IgG fractions Affinity Purified Antibodies Monoclonal Antibodies Storage of Commercial Antibodies References2. Properties of Immunoglobulins Introduction Diversity of Immunoglobulins The General Structure of Immunoglobulins (Figure 1) General Properties of Human Immunoglobulins (Table 1) Polypeptide Composition of Human Immunoglobulins (Table 2) Distribution and Characteristics of Human Immunoglobulins (Table 3) Biological Properties of Human IgG Subclasses (Table 4) Reported Antibody Specificities of Human IgG Subclasses (Table 5) Biological Properties of Human IgA Subclasses (Table 6) Human Immunoglobulin Allotypes Specificity and Frequency of Human Immunoglobulin Allotypes (Table 7) Immunoglobulins from Other Species Properties of Mouse Immunoglobulins (Table 8) Properties of Rat Immunoglobulins (Table 9) Distribution of Light Chain Types Among Various Species (Table 10) Immunoglobulin Receptors (Fc Receptors) Molecular Properties of Human Leukocyte Fc Receptors (Table 11) Distribution and Regulation of Human Leukocyte Fc Receptor Expression (Table 12) General Characteristics of Other Fc Receptors (Table 13) References3. Antibodies Recognizing Immunoglobulins The Specificity of Antibodies Recognizing Immunoglobulins of Other Species Anti-Immunoglobulin Whole Molecule Antibodies Anti-Immunoglobulin Heavy or Light Chain-Specific Antibodies Antibodies Against Immunoglobulin Subclasses Cross-Reactions of Anti-Immunoglobulin Reagents Conjugated and Derivatized Anti-Immunoglobulin Reagents Commercial Availability of Antibodies Recognizing Immunoglobulins of Different Species References4. Producing Antibodies Polyclonal Antisera Preparation of Immunogen Haptenization of Peptides Adjuvants Species and Immunization Routes Immunization Protocols Bleeding Animals and Preparation and Storage of Sera Monoclonal Antibodies 'Immune' Lymphocytes Cell fusion (Hybridoma Production) and Fusion Partners Cell Culture, Medium and Supplements Cloning Production of Monoclonal Antibodies Production of Human Monoclonal Antibodies by Lymphocyte Transformation rDNA-Produced Antibodies Spliced Immunoglobulin Molecules Recombinant DNA-Derived Immunoglobulin Fragments References5.