Gene Cloning and Manipulation
2nd Edition
Published on 24. August 2007
978-0-511-29259-0 (ISBN)
Description
Updated to reflect advances in the field, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.
More details
Edition
2nd Revised edition
Language
English
Place of publication
Cambridge
United Kingdom
Publishing group
Cambridge University Press
Target group
College/higher education
Professional and scholarly
Edition type
Revised edition
Illustrations
9 Tables, unspecified; 131 Line drawings, unspecified
File size
7,42 MB
ISBN-13
978-0-511-29259-0 (9780511292590)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
Additional editions
Christopher Howe
Gene Cloning and Manipulation
Book
07/2007
2nd Edition
Cambridge University Press
€99.20
Article exhausted; check for reprint
Christopher Howe
Gene Cloning and Manipulation
Book
07/2007
2nd Edition
Cambridge University Press
€88.40
Shipment within 15-20 days
Person
Christopher Howe is Professor of Plant and Microbial Biochemistry at the University of Cambridge. His research interests include biochemistry and molecular biology of photosynthetic organisms, and the evolutionary processes that gave rise to them. He has taught molecular biology for twenty years.
Content
Preface; 1. The tools for the job; 2. Polymerase chain reaction; 3. Simple cloning; 4. Other vector systems for E. Coli; 5. Making libraries; 6. Screening libraries; 7. Modification and mutagenesis; 8. Use of cloned DNA; 9. Using other organisms; 10. Examples.
