Theory and application of Microbiological Assay
Published on 2. December 2012
338 pages
978-0-323-15528-1 (ISBN)
Description
Theory and Application of Microbiological Assay first presents an overview of microbiological assay, including general principles, basic techniques, capabilities, and limitations. This book also explains when to use a certain microbiological assay for specific analysis, citing examples to further clarify the matter. Some chapters discuss the test organisms and solutions, wherein test solutions are the final dilutions at a series of two or more concentrations that have been prepared from reference standard and unknown for application to the test system. Other chapters explore the various aspects of agar diffusion assays, tube assays, and diffusion assays. The book further looks into the quality control procedures, assay design and evaluation, and assay replication. This book will serve as an introduction to newcomers to the field and as a reference source for established workers in microbiological assay.
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William Hewitt | S. Vincent
Theory and Applications of Microbiological Assay
Book
01/1989
Academic Press
€75.52
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Content
Preface AcknowledgmentsChapter 1 Microbiological Assay, An Overview 1.1 Introduction 1.2 General Principles of Biological Assay 1.3 Basic Techniques of Microbiological Assay 1.4 Capability and Limitations 1.5 Current Status of Antibiotic Assays 1.6 When to Use Microbiological Assays References Chapter 2 Test Organisms 2.1 Introduction 2.2 Safety 2.3 Culture Media 2.4 Freeze-Dried Assay Cultures 2.5 Culture Checks 2.6 Culture Maintenance 2.7 Preparation of the Inoculum 2.8 Preparation of Spore Suspensions 2.9 Preliminary Assay Considerations 2.10 Minimum Inhibitory Concentration References Chapter 3 Test Solutions 3.1 Introduction 3.2 Reference Standards: Principles and Problems 3.3 Reference Standards: Practical Approaches 3.4 Preparation of Test Solution References Chapter 4 The Agar Diffusion Assay 4.1 Essential Features of the Assay 4.2 Practical Methods of Zone Production 4.3 Factors Influencing Zone Size 4.4 Dose-Response Relationship 4.5 Practical Assay Designs 4 4. The Plate Method in Practice 5 References Chapter 5 Tube Assays for Growth-Inhibiting Substances 5.1 Introduction 5.2 Outline of the Method 5.3 Nature of the Response 5.4 Critical Factors 5.5 The Tube Method in Practice 5.6 Automated Methods References Chapter 6 Tube Assays for Growth-Promoting Substances 6.1 Introduction 6.2 Outline of the Method 6.3 Nature of the Response 6.4 Critical Factors 6.5 The Tube Method in Practice ReferencesChapter 7 Quality Control Procedures 7.1 Introduction 7.2 Media 7.3 Diluents 7.4 Stock Cultures 7.5 Inoculum 7.6 Spore Suspension 7.7 Working Standards 7.8 Standard Solutions 7.9 Dilutions 7.10 Plating-Out Pipettes 7.11 Miscellaneous Equipment 7.12 Designs Used in Microbiological Assays 7.13 Zone-Reading Performance 7.14 Washing Performance 7.15 Finding the Correct Dose Levels 7.16 Adjusting Zone Size 7.17 Testing Agar for Suitability in Diffusion Assays 7.18 Staff Selection and Training ReferencesChapter 8 Assay Design and Evaluation 8.1 Definitions 8.2 Dose-Response Lines: Type 1 8.3 Dose-Response Lines: Type 2 8.4 Selection of Experimental Design References Chapter 9 Diffusion Assay Methods for Antibiotics 9.1 Introduction 9A Routine Methods 9.2 Amikacin 9.3 Amoxicillin 9.4 Ampicillin 9.5 Azlocillin 9.6 Bacitracin 9.7 Benzalkonium Chloride 9.8 Carbenicillin 9.9 Cefazolin 9.10 Cefotaxime 9.11 Ceftazidime 9.12 Cefuroxime 9.13 Cephalexin 9.14 Cephaloridine 9.15 Cephalothin 9.16 Chlortetracycline 9.17 Cloxacillin 9.18 Dihydro streptomycin 9.19 Gentamicin 9.20 Neomycin 9.21 Novobiocin 9.22 Nystatin 9.23 Oxytetracycline 9.24 Penicillin G 9.25 Penicillin V 9.26 Phenylmercuric Acetate 9.27 Streptomycin 9.28 Tetracycline 9.29 Thiomersal 9.30 Tobramycin 9B Code of Federal Regulations (21 CFR) Methods 9.31 Amoxicillin 9.32 Amphomycin 9.33 Amphotericin 9.34 Ampicillin 9.35 Bacitracin 9.36 Bleomycin 9.37 Carbenicillin 9.38 Cefactor 9.39 Cefadroxil 9.40 Cefamandole 9.41 Cefazolin 9.42 Cefotaxime 9.43 Cefoxitin 9.44 Cephalexin 9.45 Cephaloglycin 9.46 Cephaloridine 9.47 Cephalothin 9.48 Cephapirin 9.49 Cephradine 9.50 Clindamycin 9.51 Cloxacillin 9.52 Colistimethate Sodium 9.53 Colistin 9.54 Cyclacillin 9.55 Dactinomycin 9.56 Dicloxacillin 9.57 Dihydrostreptomycin 9.58 Erythromycin 9.59 Gentamicin 9.60 Kanamycin 9.61 Methicillin 9.62 Mitomycin 9.63 Nafcillin 9.64 Natamycin 9.65 Neomyci 9.66 Netilmicinn 9.
