Modern Methods of Steroid Analysis

List of ContributorsPrefaceList of AbbreviationsSection I. Chromatography Chapter 1. Liquid Column Chromatography of Hormonal Steroids I. Introduction II. Recent Developments in High-Resolution Liquid Chromatography III. New Active-Shell/Inert-Core Materials for High-Resolution Chromatography IV. Liquid Chromatographs V. Application of High-Resolution, High-Speed Liquid Chromatography to Steroid Analysis VI. Application of High-Resolution, Moderate-Speed Liquid Chromatography to Steroid Analysis VII. Other High-Resolution, Moderate-Speed Methods for Liquid Chromatography of Steroids VIII. Low-Resolution Liquid Chromatography of Steroids IX. Comparison of Liquid Chromatography with Other Chromatographic Methods for Steroids X. Conclusions References Chapter 2. Application of High-Pressure Liquid Chromatography to the Separation of Insect Molting Hormones I. Introduction II. Chromatographic Theory III. Apparatus IV. Liquid-Chromatographic Separation of Ecdysones References Chapter 3. Gradient Elution and Thin-Layer Chromatography in the Analysis of Corticosteroids and 17-Ketosteroids I. Gradient Elution II. Computerized Comparison of Thin-Layer Chromatograms References Chapter 4. Gas Chromatography of Steroid Hormones I. Instrumentation II. Steroid Derivatives III. Structure and Chromatographic Behavior IV. Column Selection V. Quantitative Analysis VI. Methodology References Chapter 5. Qualitative and Quantitative Analysis of Plant Sterols by Gas-Liquid Chromatography I. Introduction II. Separation Techniques III. Gas-Liquid Chromatography IV. Results V. GLC of Other Sterol Types ReferencesSection II. Mass Spectrometry Chapter 6. Some Aspects of Mass Spectrometry in Steroid Analysis I. Introduction II. Hydrocarbons III. Monooxygenated Steroids IV. Steroids Containing More than One Oxygen Group V. Derivatives Suitable for Gas Chromatography-Mass Spectrometry VI. Quantitative Aspects of Mass Spectrometry in Steroid Analysis VII. Miscellaneous Steroids VIII. Conclusions References Chapter 7. Derivatization and Gas Chromatography in the Mass Spectrometry of Steroids I. Introduction II. Derivatization and Characterization Techniques III. Selective Detection by Mass Spectrometry IV. Possible Pitfalls ReferencesSection III. Infrared and Raman Spectroscopy Chapter 8. A Computerized Method for Rapid Comparison and Retrieval of Infrared Spectral Data I. Introduction II. The IRIS System III. Use of the Information System References Chapter 9. Raman Spectroscopy of Steroids I. Introduction II. Preparation of the Samples III. Characteristic Bonds in the Raman Spectra IV. Scheme for Steroid Structure Elucidation ReferencesSection IV. Nuclear Magnetic Resonance Chapter 10. Introduction to Nuclear Magnetic Resonance I. Introduction II. Nuclear Magnetic Resonance III. The Basic NMR Spectrometer IV. Relaxation Times and Relaxation Mechanisms V. General Features of NMR VI. Comparison of H and 13C NMR for Steroid Structural Studies VII. Sensitivity of NMR Methods References Chapter 11. Applications of Lanthanide Shift Reagents I. Introduction II. Magnetic Interactions III. Chemistry of Shift Reagents IV. Applications V. New Lanthanide Shift Reagents ReferencesSection V. X-Ray Diffraction Analysis Chapter 12. X-Ray Analysis of Steroid Structures and the Automated Diffractometer I.