ADP-Ribosylation Reactions

ContributorsPrefacePart I Introduction 1 Poly- and Mono(ADP-Ribosyl)ation Reactions: Their Significance in Molecular Biology I. Prologue II. Discovery of Poly(ADP-Ribose) III. ADP-Ribosylation of Proteins IV. Prospects ReferencesPart II ADP-Ribosylation In NAD Metabolism 2 Pyridine Nucleotide Metabolism and ADP-Ribosylation I. Introduction II. Pyridine Nucleotide Biosynthesis III. Pyridine Nucleotide Cycles IV. Summary and Conclusions ReferencesPart III Poly(ADP-Ribosyl)ation A. Structure of Poly(ADP-ribosyl) Proteins (Chapters 3-6) ¿. Poly(ADP-ribose) Biosynthesis and Degradation (Chapters 7-16) C. Natural Occurrence and Quantitation of Poly(ADP-Ribosyl) Proteins (Chapters 17-20) D. Functional Roles of Poly(ADP-Ribosyl)ation (Chapters 21-30) E. Poly(ADP-Ribose) in Medicine (Chapters 31-33) 3 Structure and Properties of Poly(ADP-Ribose) I. Structure of Poly(ADP-Ribose) II. Properties of Poly(ADP-Ribose) III. Conclusion References 4 DeoxyNAD and Poly(deoxyADP-Ribose) I. Introduction II. Synthesis and Characterization of 2'dNAD and 3'dNAD III. Template Activity of Nuclei Isolated from HeLa Cells with NAD, 2'dNAD, and 3'dNAD IV. Chain Length Determination V. ADP-Ribosylation of Isolated Rat Liver Nuclear Histone and Nonhistone Chromosomal Proteins by NAD and 2'dNAD VI. Chain Length Determination of ADP-Ribosylated Histone and Nonhistone Proteins in Isolated HeLa Nuclei Incubated with NAD or 2'dNAD VII. Characterization of the Ribose to Ribose 1" ¿ 3' Osidic Bond Formed with 2'dNAD VIII. Discussion References 5 Acceptor Proteins of Poly(ADP-Ribose) I. Introduction II. Transfer of Mono- and Poly(ADP-Ribose) to Nuclear Proteins III. Isolation of Nuclear Poly(ADP-Ribose) Protein Conjugates Synthesized in Vitro IV. Identification of Individual Nuclear Proteins Serving as Acceptors for Mono- and Poly(ADP-Ribose) V. ADP-Ribosylation of Nuclear Proteins in Vivo VI. The Effect of DNA Damage on the ADP-Ribosylation of Nuclear Proteins in Vitro and in Vivo VII. ADP-Ribosylation of Nonnuclear Proteins VIII. Concluding Remarks References 6 ADP-Ribosyl Protein Linkages I. Introduction II. ADP-Ribosylation of Histones and Other Chromosomal Proteins III. ADP-Ribosylation of Histone H1 IV. ADP-Ribosylation of Nucleosomal Histones V. Chemical Stability of the ADP-Ribosyl-Protein Linkage VI. Chemical Nature of the ADP-Ribosyl-Protein Linkages References 7 Poly(ADP-Ribose) Synthetase I. Introduction II. Nomenclature and Standard Assay III. Distribution IV. Purification V. Physicochemical Properties VI. Enzymological Properties VII. Immunological Properties VIII. Regulation of Poly(ADP-Ribose) Synthetase References 8 Poly (ADP-Ribose) Synthetase-DNA Interaction I. Introduction II. DNA Structure Required for Enzyme Activation III. Study for Enzyme-DNA Interaction by Filter Binding Assay IV. Role of Mg2+ and Polyamines in Enzyme-DNA Interaction V. Summary References 9 Chromatin Structure and Poly(ADP-Ribosyl)ation I. Chromatin Structure II. Association of Poly(ADP-Ribose) Synthetase with Polynucleosomes III. Distribution of Acceptors of Poly(ADP-Ribose) in Oligo- and Polynucleosomes IV. NAD Concentration-Dependent Influence in the Modification of Nucleoprotein Complexes V. Modulation of Chromatin Structure by Poly(ADP-Ribosyl)ation-A Model References 10 Mitochondrial ADP-Ribosyltransferase System I. Introduction II.