The Interpretation of Ultrastructure
Published on 15. July 2014
448 pages
978-1-4832-8201-5 (ISBN)
Description
The Interpretation of Ulstrastructure is a collection of papers dealing with techniques in ulstrastructural research such as in fixation, reversed staining, ultrastructure in embryos and in plants, control of observations by light microscopy, and cytochemistry at the electron microscope level. One paper describes various fixatives used on rat liver tissue including a standard osmium tetroxide solution, pure melted osmium tetroxide, and strong osmium tetroxide solutions in carbon tetrachloride. Another paper points out that the use of electron micrographs of the cell nucleus and chromosomes should include approaches to chromosome analysis. For the investigator studying the three dimensional organization of chromosomes, his approaches should include classical cytology and cytogenetics, chemical and cytochemical studies on nuclei, as well as chemical and physical analysis of nucleic acid and nucleoprotein molecules. Several papers discuss the study of natural and artificial DNA-plasma by thin sections, an assessment of negative staining techniques for revealing ultrastructure, and other approaches in the study of biological ultrastructure by high-resolution electron microscopy. The collection will benefit microbiologists, biotechnologists, and academicians connected with the biological sciences.
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Content
List of ContributorsPrefaceChemical Fixatives for Electron MicroscopyWater Soluble Embedding Media for Ultrastructural Cytochemistry. Digestion with Nucleases and ProteinasesCritical Evaluation of Ultrastructural Patterns with Respect to FixationInterpretation of Ultrastructure in the Cell NucleusCorrelation of Electron Microscope and X-Ray Diffraction Data in Ultrastructure Studies of Lipoprotein Membrane SystemsQuantitative Electron MicroscopyCytology and Microcytology of Living and Fixed Cytoplasmic Constituents in the Eggs of Tubifex and the Cell of Amoeba ProteusLight and Electron Microscopic Study of Membranous Cytoplasmic OrganellesNucleosidephosphatase Activities of CytomembranesCombination of Cytochemical Staining Methods for Enzyme Localization with Electron MicroscopyNucleoprotein Localization in Electron Micrographs: Metal Binding and RadioautographyThe Study of Natural and Artificial DNA-Plasms by Thin SectionsNegative Staining and its Use in the Study of Viruses and their Serological ReactionsAn Assessment of Negative Staining Techniques for Revealing UltrastructureThe Finer Structure of the Mitotic ApparatusInterpretation of the Ultratexture in Growing Plant Cell WallsThe Electron Microscopy and Electron Diffraction Analysis of Natural CelluloseThe Molecular Structure of Lipid-Water Systems and Cell Membrane Models Studied with the Electron MicroscopeThe Evolution of Intracellular Phospholipid Membrane SystemsRecovery of Virus from the Blood of Rats with Induced LeukaemiaElectron Microscopy in the Study of Embryonic DifferentiationNew Approaches in the Study of Biological Ultrastructure by High-Resolution Electron MicroscopyAuthor Index
