Laboratory Methods in Microbiology
Published on 28. June 2014
374 pages
978-1-4832-7434-8 (ISBN)
Description
Laboratory Methods in Microbiology is a laboratory manual based on the experience of the authors over several years in devising and organizing practical classes in microbiology to meet the requirements of students following courses in microbiology at the West of Scotland Agricultural College. The primary object of the manual is to provide a laboratory handbook for use by students following food science, dairying, agriculture and allied courses to degree and diploma level, in addition to being of value to students reading microbiology or general bacteriology. It is hoped that laboratory workers in the food manufacturing and dairying industries will find the book useful in the microbiological aspects of quality control and production development. The book is organized into two parts. Part I is concerned with basic methods in microbiology and would normally form the basis of a first year course. Abbreviated recipes and formulations for a number of typical media and reagents are included where appropriate, so that the principles involved are more readily apparent. Part II consists of an extension of these basic methods into microbiology as applied in the food manufacturing, dairying and allied industries. In this part, the methods in current use are given in addition to, or in place of, the ""classical"" or conventional techniques.
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Content
PrefacePart I Basic Methods General Notes on Laboratory Procedure Laboratory Reports Procedure for the Use of a Microscope with an Oil-Immersion Objective Examination of Cultures for Motility by "Hanging Drop" Preparations Staining Methods A. Preparation of Smears for Staining B. Simple Stains C. Gram'S Staining Method D. Ziehl-Neelsen Method for Staining Acid-Fast Bacteria E. Staining of Bacterial Flagella F. The Demonstration of Bacterial Capsules G. Negative Stain H. The Staining of Bacterial Spores Cultivation of Micro-Organisms A. Types of Culture B. Incubation of Cultures C. Method of Inoculation: Aseptic Technique D. Maintenance of Pure Cultures in the Laboratory The Description of the Morphological and Cultural Characteristics of an Organism A. Morphological Characters B. Cultural Characters Plate Cultures A. Preparation of Plates for Streaking B. Streak Plates: The Separation of Mixed Cultures Determination of the Number of Viable Organisms in a Sample A. Colony Count Methods B. Membrane Filtration C. Dilution Tube Count D. Dye Reduction Methods Determination of the Total Number of Organisms in a Sample A. the Breed's Smear Method for Direct Microscopic Counts B. Direct Microscopic Counts by Membrane Filtration C. Brown's Opacity Tubes Statistical Methods for the Selection and Examination of Microbial Colonies Composition of Culture Media A. Introduction B. Dehydrated Media C. The Determination of the pH of Culture Media D. Examples of Non-Selective Culture Media E. Separation of Mixed Cultures-Enrichment Procedures, Elective and Selective Media F. Examples of Selective Culture Media Methods of Anaerobic Culture A. Robertson's Cooked Meat Medium B. Shake Cultures C. Semi-Solid Media D. Vaseline, Paraffin Wax or Agar Seals E. The Anaerobic Jar Cultivation in a Carbon Dioxide-Enriched Atmosphere Biochemical Tests for Bacteria A. Reactions Involving Protein, Amino Acids and Other Nitrogen Compounds, Including Tests for Proteolytic Activity B. Reactions Involving Carbohydrates and Other Carbon Compounds, Including Tests for Saccharolytic Activity C. Reactions Involving Fats and Related Substances, Including Tests for Lipolytic Activity D. Miscellaneous Tests Cleaning of Glassware and Apparatus A. Treatment of New Glassware B. Treatment of Used Glassware and Other Apparatus C. Disposable Apparatus Sterilization A. Sterilization by Dry Heat B. Sterilization by Moist Heat C. Sterilization by Filtration D. Chemical Disinfectants E. Preparation of Glassware and Materials for Sterilization F. To Test the Sterility of Laboratory Equipment Laboratory Evaluation of Disinfectants A. The Rideal-Walker Test B. The Suspension Test C. The Capacity Test Serological Methods A. Introduction B. The Agglutination Reaction C. The Precipitin Test Molds and Yeasts A. General Conditions for the Growth of Molds and Yeasts B. Media for the Growth of Molds and Yeasts C. Examination of Molds D. Examination of Yeasts E. Note on the Identification of Molds and Yeasts The Isolation of BacteriophagePart II Techniques in Applied Microbiology The Bacteriological Examination of Water A. Introduction B. Sampling Procedure C. Plate Counts D. Presumptive Coliform Counts and the Eijkman Test E. Enumeration of Fecal Streptococci F. Detection of Clostridium Welchii G.
