Proteomics Today
Protein Assessment and Biomarkers Using Mass Spectrometry, 2D Electrophoresis,and Microarray Technology
Published on 29. April 2005
448 pages
978-0-471-70910-7 (ISBN)
Description
The last few years have seen an unprecedented drive toward theapplication of proteomics to resolving challenging biomedical andbiochemical tasks. Separation techniques combined with modern massspectrometry are playing a central role in this drive. This bookdiscusses the increasingly important role of mass spectrometry inproteomic research, and emphasizes recent advances in the existingtechnology and describes the advantages and pitfalls as well.
* Provides a scientifically valid method for analyzing theapproximatey 500,000 proteins that are encoded in the humangenome
* Explains the hows and whys of using mass spectrometry inproteomic analysis
* Brings together the latest approaches combining separationtechniques and mass spectrometry and their application in proteomeanalysis
* Comments on future challenges and how they may be addressed
* Includes sections on troubleshooting
Reviews / Votes
"I highly recommend this book for anyone planning to get involvedin proteomics technology or for one who is alreadyinvolved...it provides an entertaining insight on the field..."(Microbe, March 2006) "...a must reference for anyone interested in proteomics. Forme, if Proteomics Today is not in my backpack it will be withinarms reach as a great reference to have on hand as I plan futureexperiments." (Journal of the American Society for MassSpectrometry, January 2006) "...the book is highly recommended to all scientistsinterested in protein separation science, and well-thumbed copieswill certainly be present in every self-respecting proteomiclaboratory." (Proteomics, July 2005) 'This book is well written and thus easy to read. Itcontains valuable information and extensive references for readersfrom a variety of backgrounds.' (Analytical andBioanalytical Chemistry, 12 January 2007) "...well-written and thus easy to read. It contains valuableinformation and extensive references for readers from a variety ofbackgrounds." (Analytical and Bioanalytical Chemistry,January 2007)More details
Series
Edition
1. Auflage
Language
English
Place of publication
Newark
United States
Publishing group
John Wiley & Sons
Illustrations
Charts: 0 B&W, 0 Color; Photos: 0 B&W, 0 Color; Drawings: 0 B&W, 0 Color; Tables: 0 B&W, 0 Color; Graphs: 0 B&W, 0 Color
File size
5,64 MB
ISBN-13
978-0-471-70910-7 (9780471709107)
Schweitzer Classification
Other editions
Additional editions
Software
05/2005
Wiley
€209.83
Shipment within 10-20 days
Mahmoud H. Hamdan | Pier G. Righetti | Dominic M. Desiderio
Proteomics Today
Protein Assessment and Biomarkers Using Mass Spectrometry, 2D Electrophoresis, and Microarray Technology
Book
03/2005
Wiley
€166.50
Shipment within 10-20 days
Persons
MAHMOUD HAMDAN, PhD, is the head of Mass Spectrometry &Separation Technologies, GlaxoSmithKline, Verona, Italy.
PIER GIORGIO RIGHETTI, PhD, is Professor of Biochemistry anddirector of the Laboratory of Proteome Science at the University ofVerona, Italy.
Content
PREFACE TO PART I.
ACKNOWLEDGMENT.
I: INTRODUCTION TO PART I.
1. INSTRUMENTATION AND DEVELOPMENTS.
1.1 Introduction.
1.2 Ionization Techniques for Macromolecules.
1.3 Examples on Analytical Solutions Based on FAB-MS.
1.4 Electrospray Ionization.
1.5 Matrix-Assisted Laser Desorption Ionization.
1.6 Ion Detection.
1.7 Types of Analyzers.
1.8 Hybrid Analyzers.
1.9 Tandem Mass Spectrometry.
1.10 Current MS Instrumentation in Proteome Analyses.
1.11 Current MS-Based Proteomics.
1.12 Recent Achievements and Future Challenges.
1.13 Concluding Remarks.
References.
2. PROTEOMICS IN CANCER RESEARCH.
2.1 Introduction.
2.2 Pancreatic Ductal Adenocarcinoma.
2.3 Proteomic Analysis of Human Breast Carcinoma.
2.4 Proteomic Profiling of Chemoresistant Cancer Cells.
2.5 Signal Pathway Profiling of Prostate Cancer.
2.6 Emerging Role of Functional and Activity-Based Proteomics inDisease Understanding.
2.7 Activity-Based Protein Profiling.
2.8 Probing Protein Functions Using Chromophore-Assisted LaserInactivation.
2.9 Role of Protein-Tyrosine Kinases.
2.10 Concluding Remarks and Future Prospects.
References.
3. CURRENT STRATEGIES FOR PROTEIN QUANTIFICATION.
3.1 Introduction.
3.2 Global Internal Standard Technology.
3.3 Differential In-Gel Electrophoresis.
3.4 Quantification of Modified Proteins.
3.5 Comments and Considerations.
3.6 Other Approaches.
3.7 Emerging Role of Microfluidic Devices.
3.8 Concluding Remarks.
References.
II: PROTEOMICS TODAY: SEPARATION SCIENCE AT WORK.
4. CONVENTIONAL ISOELECTRIC FOCUSING IN GEL MATRICES ANDCAPILLARIES AND IMMOBILIZED pH GRADIENTS.
4.1 Introduction.
4.2 Conventional Isoelectric Focusing in Amphoteric Buffers.
4.3 Immobilized pH Gradients.
4.4 Capillary Isoelectric Focusing.
4.5 Separation of Peptides and Proteins by CZE in IsoelectricBuffers.
4.6 Conclusions.
References.
5. SODIUM DODECYL SULFATE-POLYACRYLAMIDE GELELECTROPHORESIS.
5.1 Introduction.
5.2 SDS-Protein Complexes: a Refinement of the Model.
5.3 Theoretical Background of Mr Measurement bySDS-PAGE.
5.4 Methodology.
5.5 Gel Casting and Buffer Systems.
5.6 Blotting Procedures.
5.7 Conclusions.
References.
6. TWO-DIMENSIONAL MAPS.
6.1 Introduction.
6.2 Some Basic Methodology Pertaining to 2D PAGE.
6.3 Prefractionation Tools in Proteome Analysis.
6.4 Multidimensional Chromatography Coupled to MS.
6.5 Protein Chips and Microarrays.
6.6 Nondenaturing Protein Maps.
6.7 Spot Matching in 2D Gels via Commercial Software.
6.8 Conclusions.
References.
INDEX.
