HPTLC - High Performance Thin-Layer Chromatography
Elsevier (Publisher)
Published on 26. August 2011
231 pages
978-0-08-085809-8 (ISBN)
Description
HPTLC - High Performance Thin-Layer Chromatography
More details
Other editions
Additional editions
Albert Zlatkis | R.E. Kaiser
High Performance Thin-layer Chromatography
Book
11/1976
Elsevier
€33.43
Article exhausted; check different version
Persons
Content
- Front Cover
- HPTLC High Performance Thin-Layer Chromatography
- Copyright Page
- Table of Contents
- Preface and Introduction
- Nomenclature
- Chapter 1. Simplified theory of TLC
- Rf-value and k, Real Rf-values
- Flow function in HPTLC, Rf-value in HPTLC
- Correlation between circular HPTLC Rf and linear Rf-values
- Separation capability and separation power in HPTLC
- Plate height and separation number in TLC
- Resolution, selectivity, separation capability
- Conclusions
- Chapter 2. The separation number in linear and circular TLC
- Calculation of the separation number in linear TLC
- The space diffusion model
- Determination of the separation number in circular TLC
- Critical questions
- Chapter 3. Advantages, limits and disadvantages of the ring developing technique
- Prerequisits for the ring developing technique
- Working technique in circular chromatography
- Comparison of the methods linear TLC and circular TLC(CTCL)
- Ratio of Rf to RRf
- Gradient effect
- Reduction of vapor pressure
- Flowing around effect
- Dynamic area,Trace analysis
- Effect of solvent atmosphere
- Material required
- Space required, handling ability, miscellaneous
- Chapter 4. The U-chamber
- Advantages
- Disadvantages of the normal U-chamber system, Conclusions
- Comparison of migration speed
- Principle of wet dosage in HPTLC
- Continuous HPTLC
- Coupling with another separation or detection systems
- Plate adjustment and dosage
- Chapter 5. Dosage techniques in HPTLC
- Primary optimized dosage
- Dosage volume
- Recommendations
- Instrumentation for nano dosage, Self loading sample capillary
- Glass-metal capillary
- Constant dosage capillary
- Sample application on the wet layer
- Thermal and chemical focusing
- Chapter 6. High performance thin-layer chromatography: development, data and results
- Introduction
- Development of the pre-coated HPTLC plate
- Chromatographic performance of the HPTLC plate
- Chromatographic characteristics
- Influences of the type of chamber and of sorbent and solvent activity
- Influence of temperature
- Differences between linear and circular chromatography
- Advantages of HPTLC
- Chromatographic and physical parameters of solvents, elutropic series breakdown related to silicagel
- Chapter 7. Consideration on the reproducibility of TLC separations
- Experimental
- Modification of the stationary phase by substances other than components of the developing solvent
- Influence of layer preconditioning with components of the developing solvent
- Conclusion
- The role of solvent pre-adsorbed by the dry layer
- Conclusions
- Transfer TLC-HPLC: Compatible experimental approach
- is equilibration via the gas phase comparable to liquid phase equilibration?
- Results
- Chapter 8. Potential and experience in quantitative HPTLC evaluation
- Introduction
- Application techniques
- Photometric procedures for HPTLC evaluation
- Absorption measurements in reflectance and transmission mode
- Smoothing of baselines by simultaneous reflectance and transmission measured
- Absorption measurements in UV and fluorescence "quenching"
- Fluorescence measurements
- Reproducibility
- Qualitative initial examinations
- Practical examples
- Requirements of the instrument for the photometric evaluation of HPTLC chromatograms
- Chapter 9. Application of a new high-performance layer in quantitative TLC
- Advantages of HPTLC precoated plates
- Applicator for TLC
- Apparatus for nanoliter application
- Peak area and baseline determination
- Evaluation procedures
- Wavelength optimization
- Comparison of the detection at right-angles to solvent flow and in flow direction
- Limit of detection, Detection of phenylanaline in serum
- Regression line and correlation coefficient
- Aflatoxine measurements
- Coloured figures
- Principle of simultaneous three phase circular separation in a IfC-U-Chamber
- Simultaneous multiphasecircular HPTLC of dye mixture
- Detection of trace level actions
- Power of two dimensional HPTLC on 50 x 50 mm plates
- Separation power and Rf data precision of HPTLC layers
- 40 samples simultaneously separated in one run
- 50 x 50 mm circular HPTLC for precise qualitative and quantitative analysis
- Appendix
- Conversion table Rf to k and k to Rf values.
- Pocket computer program for linear regression calculation
- Index
