Methods of Animal Experimentation

List of ContributorsPrefaceContents of Volume I IntroductionChapter 1 Radiation Hygiene I. The Nature and Assessment of Ionizing Radiation Effects II. Health Physics Appendix ReferencesChapter 2 Use of Ionizing Radiation for Measuring Biological Phenomena I. Introduction II. Laboratory Design III. Handling of Radioisotopes in the Animal Colony IV. Administration of Radiation or Radioisotopes to the Experimental Animal V. Collection of Samples VI. Uses of Radioisotopes Appendix ReferencesChapter 3 Tumor Transplantation I. Introduction II. History and Biological Properties of 38 Transplantable Mouse, Rat, Hamster, and Chicken Tumors III. Techniques of Tumor Transplantation IV. Importance of Uniform Inoculum V. Importance of Animal Strain VI. Inoculation Site VII. Influence of Age and Sex VIII. Influence of Health IX. Influence of Pregnancy X. Interval after Which Growth Becomes Apparent XI. Comparative Growth Rate of the Malignant Cells XII. Inoculation of Stationary or Receding Tumors XIII. Spontaneous Regression of Transplantable Tumors XIV. Resistance to Reinoculation XV. Frozen Storage of Transplantable Tumors XVI. Summary ReferencesChapter 4 Parabiosis I. Introduction II. Methods III. Blood and Fluid Exchange Between Parabiotic Rats IV. Diseases of Parabiosis ReferencesChapter 5 Organ Transplantation I. General Discussion of the Types of Problem That Can Be Investigated by Experimental Organ Transplantation II. The Selection of Animals for Organ Transplantation III. The Surgical Technique IV. Methods of Assessment of Renal Function and Homograft Reaction in Animals with Renal Transplants V. Summary ReferencesChapter 6 The Care and Use of Hibernating Mammals I. Hibernation II. Mammals That Hibernate III. Types of Mammalian Hibernation IV. Environmental Factors V. Hibernation versus Hypothermia VI. Estivation VII. Choice of Animal for Research VIII. Procurement of Animals IX. Care of Individual Species X. Techniques for Use of Hibernators ReferencesChapter 7 High-Altitude Studies I. Introduction II. "Natural" High-Altitude Studies III. "Simulated" High-Altitude Studies IV. Conclusions ReferencesChapter 8 Care and Growth of Animals during Chronic Centrifugation I. Introduction II. Applications III. General Considerations in the Design of Centrifuge Programs IV. Specific Procedures Employed with Experimental Animals V. Procedures of Measurement and Analysis VI. Summary ReferencesChapter 9 Controlled Exercise I. Introduction II. Methods of Inducing Controlled Exercise III. Factors Influencing Controlled Exercise Results IV. Factors Affecting Endurance VI Influence of Excess Exercise on Various Animal Characteristics VI. Summary ReferencesChapter 10 Methods of Animal Infection with Bacteria, Fungi, and Viruses I. Introduction II. Animal Inoculation versus Tissue Culture Testing and the Use of Artificial Media III. Factors Influencing the Choice of Animals and Route of Inoculation IV. General Procedures According to Route of Inoculation V. Study of Specific Infections ReferencesChapter 11 Electronic Monitoring of Physiological Phenomena in Experimental Animals I. Components of a Monitoring System II. Electrocardiography III. Intravascular and Intracardiac Electrocardiography IV. Phonocardiography V. Intravascular Pressures VI. Ultrasound VII. Temperature Monitoring VIII. Respiration IX. Densitometry X. Electrophysiological Signals from the Brain ReferencesAuthor IndexSubject Index