Microglia
Methods and Protocols
Published on 7. August 2019
XII, 340 pages
978-1-4939-9658-2 (ISBN)
Description
This book presents a comprehensive toolkit of versatile techniques for studying microglia under different experimental settings along with a brief summary of knowledge, accumulated in microglial research over the last decades. Beginning with recently discovered roles of microglia in health and disease, the volume continues by covering
in vitro
analyses of microglia,
in vivo
studies, and "omics" analyses. Written for the highly successful
Methods in Molecular Biology
series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Microglia: Methods and Protocols serves as a vital guide to these important cells and an inspiration for scientists interested in expanding our knowledge of their role in the nervous system.
Authoritative and cutting-edge, Microglia: Methods and Protocols serves as a vital guide to these important cells and an inspiration for scientists interested in expanding our knowledge of their role in the nervous system.
More details
Series
Language
English
Place of publication
New York
United States
Publishing group
Springer US
Target group
Professional and scholarly
Product notice
Reflowable
Illustrations
XII, 340 p. 65 illus., 6 illus. in color.
File size
10,49 MB
ISBN-13
978-1-4939-9658-2 (9781493996582)
DOI
10.1007/978-1-4939-9658-2
Schweitzer Classification
Other editions
Additional editions
Book
08/2019
Humana Press Inc.
€181.89
Shipment within 15-20 days
Content
Microglia: The Neural Cells of Non-Neural Origin.- Morphology of Microglia across Contexts of Health and Disease.- Physiology of Microglia.- Microglia Reactivity: Heterogeneous Pathological Phenotypes.- Microglia in Neurodegenerative Disorders.- Physiological Implications of Microglia-Synapse Interactions.- Ultrastructural Analyses of Microglial Interactions with Synapses.- Visualizing Dark Microglia.- Electrophysiological Investigation of Microglia.- Replenishment of Organotypic Hippocampal Slice Cultures with Neonatal or Adult Microglia.- Assessment of Parasite-Microglia Interactions In Vitro.- In Vivo Visualization of Microglia Using Tomato Lectin.- Long-Term In Vivo Imaging of Individual Microglial Cells.- Analysis of Signaling Mechanisms Regulating Microglial Process Movement.- Measuring Microglial Turnover in the Adult Brain.- Novel Microglia Depletion Systems: A Genetic Approach Utilizing Conditional Diphtheria Toxin Receptor Expression and a Pharmacological Model Based on the Blocking of Macrophage Colony-Stimulating Factor 1 Receptor.- Single Cell Electroporation for Measuring In Vivo Calcium Dynamics in Microglia.- Labeling Microglia with Genetically-Encoded Calcium Indicators.- Calcium Imaging of Microglial Network Activity in Stroke.- In Vivo Two Photon Imaging of Microglial Synapse Contacts.- In Vivo Phagocytosis Analysis of Amyloid Beta.- Functional Analysis of Brain-Engrafted Monocytes after Microglial Ablation in Mouse Models.- Analysis of the Microglial Sensome.- Transcriptomics and Live Imaging to Define Functional Phenotypes of Microglia in Pathological Human Tissue.
