MCB: CAR T Cells: Development, Characterization and Applications
Published on 11. February 2022
242 pages
978-0-323-85502-0 (ISBN)
Description
MCB: CAR T Cells: Development, Characterization and Applications, Volume 167 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including High-efficiency of genetic modification using CRISPR/Cpf1 system for engineered CAR T-cell therapy, Determination of the Biodistribution of Chimeric Antigen Receptor-Modified T Cells against CD19 in NSG Mice, Generation of CAR-T cells using lentiviral vectors, Generation of CAR T-cells using ¿-retroviral vector, Flow cytometry detection and quantification of CAR T cells into solid tumors, Evaluation of CAR-T Cell Cytotoxicity: Real-Time Impedance-Based Analysis, and much more.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Cell Biology series
- Includes the latest information on the topic of development, characterization and applications in CAR T Cells
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Lorenzo Galluzzi | Sheila Spada
MCB: CAR T Cells: Development, Characterization and Applications: Volume 167
Book
02/2022
Academic Press
€172.08
Shipment within 15-20 days
Content
1. High-efficiency of genetic modification using CRISPR/Cpf1 system for engineered CAR T-cell therapyRenpeng Ding, Cheng-Chi Chao and Qianqian Gao2. Determination of the biodistribution of chimeric antigen receptor-modified T cells against CD19 in NSG mice Hairuo Wen, Ying Huang, Tiantian Hou, Junzhi Wang and Yan Huo3. Generation of CAR-T cells using lentiviral vectorsMansour Poorebrahim, Isaac Quiros-Fernandez, Elham Fakhr and Angel Cid-Arregui4. Phenotyping of CAR T cellsSanjay Rathod5. Evaluation of CAR-T cell cytotoxicity: Real-time impedance-based analysisAmanda N. Lisby, Robert D. Carlson, Trevor R. Baybutt, Megan Weindorfer and Adam E. Snook6. Flow cytometry detection and quantification of CAR T cells into solid tumorsNicholas J.A. Tokarew, Javier Suárez Gosálvez, Alessia Nottebrock, Daria Briukhovestka, Stefan Endres, Bruno L. Cadilha and Sebastian Kobold7. Generation and functional characterization of CAR exosomesChuqi Wang, Wenyan Fu, Changhai Lei and Shi Hu8. Preclinical testing of CAR T cells in zebrafish xenograftsSarah Grissenberger, Benjamin Salzer, Susana Pascoal, Andrea Wenninger-Weinzierl, Manfred Lehner and Martin Distel9. In vivo experimental mouse model to test CD19 CAR T cells generated with different methodsAnne Fåne, Marit Renée Myhre, Else Marit Inderberg and Sébastien Wälchli10. Non-invasive fluorescence imaging for tracking immune cells in preclinical models of immunotherapyMarcelo de Souza Fernandes Pereira, Aarohi Thakkar and Dean Anthony Lee11. Generation of CAR T-cells using ¿-retroviral vectorNorihiro Watanabe and Mary Kathryn McKenna12. Methods to monitor in vivo expansion and efficacy of CAR-T cells in preclinical modelsMohammad Alhomoud, Jeremie Martinet, Mayumi Sugita, Alexandra Gomez-Arteaga and Monica L. Guzman13. Strategies for manufacturing cell therapy products aligned with patient needsPrajna Guha and Steven C. Katz
