Flow Cytometry

Name: Flow Cytometry
Brand: Academic Press
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Academic Press

Published on 28. January 1991

715 pages

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978-0-08-085931-6 (ISBN)

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Front Cover
Methods in Cell Biology: Flow Cytometry
Copyright Page
Contents
Contributors
Preface
Chapter 1. Dissociation of Cells from Solid Tumors
Chapter 2. Flow Cytometric Measurement of Cell Viability Using DNase Exclusion
Chapter 3. Cell Cycle Phase-Specific Analysis of Cell Viability Using Hoechst 33342 and Propidium Iodide after Ethanol Preservation
Chapter 4. Cell Membrane Potential Analysis
Chapter 5. Flow Cytometric Measurement of Intracellular Ionized Calcium in Single Cells with Indo-l and Fluo-3
Chapter 6. Measurement of Intracellular pH
Chapter 7. Flow Cytometric Techniques for Measurement of Cytochrome P-450 Activity in Viable Cells
Chapter 8. Fluorescent Staining of Enzymes for Flow Cytometry
Chapter 9. Supravital Cell Staining with Hoechst 33342 and DiOC5(3)
Chapter 10. Specific Staining of DNA with the Fluorescent Antibiotics, Mithramycin, Chromomycin, and Olivomycin
Chapter 11. DAPI Staining of Fixed Cells for High-Resolution Flow Cytometry of Nuclear DNA
Chapter 12. High-Resolution DNA Measurements Using the Nuclear Isolation Medium, DAPI, with the RATCOM Flow Cytometer
Chapter 13. Rapid DNA Content Analysis by the Propidium Iodide-Hypotonic Citrate Method
Chapter 14. An Integrated Set of Methods for Routine Flow Cytometric DNA Analysis
Chapter 15. DNA Analysis from Paraffin-Embedded Blocks
Chapter 16. Detection of M and Early-Gl Phase Cells by Scattering Signals Combined with Identification of G1, S, and G2 Phase Cells
Chapter 17. Controls, Standards
and Histogram Interpretation in DNA Flow Cytometry
Chapter 18. Cell Division Analysis Using Bromodeoxyuridine-Induced Suppression of Hoechst 33258 Fluorescence
Chapter 19. Cell Cycle Analysis Using Continuous Bromodeoxyuridine Labeling and Hoechst 33258-Ethidium Bromide Bivariate Flow Cytometry
Chapter 20. Detection of Bromodeoxyuridine-Labeled Cells by Diffeential Fluorescence Analysis of DNA Fluorochromes
Chapter 21. Using Monoclonal Antibodies in Bromodeoxyuridine-DNA Analysis
Chapter 22. Identification of Proliferating Cells by Ki-67 Antibody
Chapter 23. Washless Double Staining of a Nuclear Antigen (Ki-67 or Bromodeoxyuridine) and DNA in Unfixed Nuclei
Chapter 24. Analysis of Proliferation-Associated Antigens
Chapter 25. The Stathmokinetic Experiment: A Single-Parameter and Multiparameter Flow Cytometric Analysis
Chapter 26. Detection of Intracellular Virus and Viral Products
Chapter 27. Differential Staining of DNA and RNA in Intact Cells and Isolated Cell Nuclei with Acridine Orange
Chapter 28. Flow Cytometric Analysis of Double-Stranded RNA Content Distributions
Chapter 29. Simultaneous Fluorescent Labeling of DNA, RNA, and Protein
Chapter 30. Flow Cytometric Cell-Kinetic Analysis by Simultaneously Staining Nuclei with Propidium Iodide and Fluorescein Isothiocyanate
Chapter 31. Flow Cytometric Methods for Studying Isolated Nuclei: DNA Accessibility to DNase I and Protein-DNA Content
Chapter 32. Acid-Induced Denaturation of DNA in Situ as a Probe of Chromatin Structure
Chapter 33. Fluorescent Methods for Studying Subnuclear Particles
Chapter 34. Chromosome and Nuclei Isolation with the MgSO4 Procedure
Chapter 35. Univariate Analysis of Metaphase Chromosomes Using the Hypotonic Potassium Chloride-Propidium Iodide Protocol
Chapter 36. Polyamine Buffer for Bivariate Human Flow Cytogenetic Analysis and Sorting
Chapter 37. Fluorescence in Situ Hybridization with DNA Probes
Chapter 38. Flow Cytometric Analysis of Male Germ Cell Quality
Chapter 39. Cell Preparation for the Identification of Leukocytes
Chapter 40. Multiparameter Analysis of Leukocytes by Flow Cytometry
Chapter 41. Identification and Purification of Murine Hematopoietic Stem Cells by Flow Cytometry
Chapter 42. Fluorescent Cell Labeling for in Vivo and in Vitro Cell Tracking
Chapter 43. Rapid Determination of Cellular Resistance-Related Drug Efflux in Tumor Cells
Chapter 44. Slit-Scan Flow Analysis of Cytologic Specimens from the Female Genital Tract
Chapter 45. Cell Sorting with Hoechst or Carbocyanine Dyes as Perfusion Probes in Spheroids and Tumors
Chapter 46. DNA Measurements of Bacteria
Chapter 47. Isolation and Flow Cytometric Characterization of Plant Protoplasts
Chapter 48. Flow Cytometric Analysis of Plant Genomes
Chapter 49. Plant Cell Cycle Analysis with Isolated Nuclei
Chapter 50. Environmental Health: Flow Cytometric Methods to Assess Our Water World
Chapter 51. Flow Microsphere Immunoassay for the Quantitative and Simultaneous Detection of Multiple Soluble Analytes
Chapter 52. On-Line Flow Cytometry: A Versatile Method for Kinetic Measurements
Chapter 53. Calibration of Flow Cytometer Detector Systems
Chapter 54. Spectral Properties of Fluorochromes Used in Flow Cytometry
Index
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Flow Cytometry

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