
The Enzymes
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Content
- Front Cover
- The Enzymes: Nucleic Acids
- Copyright Page
- Contents
- List of Contributors
- Preface
- Section I. Ligases
- Chapter 1. DNA Ligases
- I. Introduction
- II. Isolation and Physical Properties
- III. Catalytic Properties
- IV. Role of DNA Ligases in Vivo
- V. Research Applications
- Chapter 2. T4 RNA Ligase
- I. Introduction
- II. Purification and Properties
- III. Reactions Catalyzed
- IV. Biological Role
- V. Applications
- Section II. RNA Polymerares and Related Enzymes
- Chapter 3. Bacterial DNA-Dependent RNA Polymerases
- I. Background
- II. Molecular Properties
- III. Catalytic Properties
- Chapter 4. Bacteriophage DNA-Dependent RNA Polymerases
- I. Introduction
- II. T7-Like RNA Polymerases
- III. Other Bacteriophage RNA Polymerases
- Chapter 5. Eukaryotic RNA Polymerases
- I. Introduction
- II. Purification
- III. Subunit Structures
- IV. Subunit Functions
- V. Stimulatory Factors
- VI. DNA Binding and Catalytic Properties of Purified RNA Polymerases
- VII. Inhibitors
- VIII. Eukaryotic Transcription Extract Systems
- IX. Organelle- and Viral-Coded RNA Polymerases
- Chapter 6. Priming Enzymes
- I. Introduction
- II. dnaG Gene Product from Escherichia coli
- III. Multiple Pathways for dnaG Priming in SS to RF DNA Replication
- IV. Studies on the Specificity of dnaG-Template Interactions
- V. Priming by RNA Polymerases
- VI. Priming on Double-Stranded DNA
- VII. Phage-Encoded Priming Enzymes
- VIII. Priming in Eukaryotic Systems
- Chapter 7. tRNA Nucleotidyltransferase
- I. Introduction
- II. Purification and Structural Studies
- III. Catalytic Properties
- IV. Biological Role
- V. Research Applications
- Chapter 8. Poly(A) Adding Enzymes
- I. Introduction and Perspective
- II. Purification and Properties
- III. Multiple Poly(A) Polymerases
- IV. Properties of Poly(A) Polymerase Proteins
- V. Assay
- VI. Stoichiometry
- VII. Substrates
- VIII. Primer Requirement
- IX. Ion Requirements
- X. Inhibitors
- XI. Kinetics and Reaction Mechanism
- XII. Biological Role
- XIII. Regulation of Poly(A) Polymerases
- XIV. Research Applications
- Chapter 9. Capping Enzyme
- I. Introduction
- II. Vaccinia Virus Capping Enzymes
- III. HeLa Cell Capping Enzymes
- IV. Capping Enzyme from Rat Liver Nuclei
- V. Role of the Capping Enzyme System in Vivo
- VI. Research Applications of Vaccinia Virus Capping Enzyme
- Chapter 10. Qß Replicase
- I. Introduction
- II. Purification and Properties
- III. Catalytic Properties
- Chapter 11. 2',5'-Oligoadenylate Synthetase
- I. Introduction
- II. Purification and Properties
- III. Reactions Catalyzed
- IV. Biological Role
- Section III. RNA Nucleases and Related Enzymes
- Chapter 12. Pancreatic Ribonuclease
- I. Introduction
- II. Preparation
- III. Chemical Properties
- IV. Physical Properties
- V. Species Variations
- VI. Bovine Seminal Plasma RNase
- VII. Cytoplasmic RNase Inhibitor
- VIII. Catalytic Properties
- IX. Research Applications
- Chapter 13. Ribonuclease T1
- I. Introduction
- II. Purification and Chemical Properties
- III. Reactions Catalyzed
- IV. Research Applications
- V. Other Guanine-Specific RNases
- Chapter 14. tRNA Processing Enzymes from Escherichia coli
- I. Introduction
- II. Ribonuclease P
- III. Ribonuclease III
- IV. Ribonuclease P2 and Ribonuclease O
- V. Ribonuclease D
- VI. Other Nucleases
- VII. Concluding Remarks
- Chapter 15. Ribonuclease III
- I. Introduction
- II. Purification
- III. Structure
- IV. Cleavage of Double-Stranded RNA
- V. Cleavage of Single-Stranded RNA
- VI. Related Enzymes from Eukaryotes
- Chapter 16. RNases I, II, and IV of Escherichia coli
- I. Introduction
- II. RNase I of Escherichia coli
- III. RNase II of Escherichia coli
- IV. RNase IV of Escherichia coli
- Chapter 17. Polynucleotide Phosphorylase
- I. Introduction
- II. Properties
- III. The Reactions Catalyzed
- IV. Attributed Physiological Functions
- V. Research Applications
- Section IV. RNA Modification
- Chapter 18. RNA Methylation
- I. Introduction
- II. Structures and General Assay Procedure
- III. Specific tRNA Methyltransferase Enzymes
- IV. Conclusion
- Chapter 19. Nucleotide Modification in RNA
- I. Introduction
- II. Modification of Uridine
- III. Modification of Cytidine
- IV. Modification of Adenosine
- V. Modification of Guanosine
- VI. Conclusion
- Author lndex
- Subject Index
- Contents of Other Volumes
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