RNA Interference

Transgene-induced RNA Interference as a Tool for Plant Functional GenomicsConstructs and Methods for hairpin RNA-mediated Gene Silencing in PlantsRNA interference in Caenorhabditis elegansHigh-throughput RNAi screens in Drosophila tissue culture cellsMechanistic insights aid computational siRNA design Novel methods for expressing RNAi in human cellsDelivery of Small Interfering RNA to Mammalian Cells in Culture Using Cationic Lipid/Polymer-Based Transfection ReagentsThe subcellular distribution of siRNA: directed delivery through RNA polymerase III expression cassettes nd localization using in situ hybridization.Viral delivery of recombinant shRNAsDownregulation of cellular genes by PCR cassettes expressing short interfering RNAsUsing short hairpin RNA expression vectors to study mammalian neural developmentAnalysis of T-cell development using siRNA to knockdown protein expressionViral delivery of recombinant siRNA expression: lentivirusParadigms for Conditional Expression of RNAi Molecules for use Against Viral TargetsHigh throughput RNAi strategies for target discovery and validation using synthetic siRNAs: functional genomics investigations of biological pathwaysRNA interference in vivo: towards synthetic siRNA based therapeuticsLabeling and characterization of small RNAs associated with the RNAi effector complex, RITSRNAi spreading in C. elegansHuman Dicer: Purification, properties, and interaction with PAZ PIWI domain proteinsDelivery of siRNA and siRNA Expression Constructions to Adult Mammals Using Hydrodynamic Intravascular InjectionAnalysis of siRNA function in RNAi using Drosophila Embryo extracts and Schneider cellsUsing RNA polymerase II to transcribe artificial microRNAsAdeno-associated virus vectors for short hairpin RNA expression Simple, Robust Strategies for Generating ddRNAi Constructs