
Bioprinting
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Content
- cover
- Half title
- Bioprinting
- Copyright
- Dedication
- Contents
- A Note to the Reader
- Foreword
- Preface
- Acknowledgments
- Introduction: Frailty, Thy Name Is Human
- 1. Printing Paradigms
- Automated Printing
- 3D Printing-?The Foundation of Bioprinting
- 3D Printing Techniques
- Trailblazers of Bioprinting
- Medical Successes of 3D Printing
- Transitioning from 3D Printing to Bioprinting
- Bioreactors-?A Boarding School Where Bioprinted Constructs Go to Mature
- Bioinks-?The Write Stuff
- Extracellular Matrices-?The Places Cells Call Home
- Rheology-?It's Flow Time
- Scaffolds
- A Return to Paradigm Shifts
- 2. All About Cells
- Mapping out Our Tour
- Cell Surface Membrane
- Cell Membrane Proteins
- Cell Factories
- Becoming a Cell-?Stem Cells
- Induced Pluripotent Stem Cells
- Additional Sources of Stem Cells
- Progenitor Cells
- The Different Cells in Our Body
- The Human Cell Atlas
- Cells Live in Three Dimensions-?Not Two Dimensions
- Cells Talk
- "When Is a Kidney a Kidney?"
- Moore's Law
- 3. Bioprinted Cartilage: The Dream and the Devilish Details
- Articular Cartilage-?A Heavily Challenged Tissue
- Bioprinted Hydrogels with Articular Cartilage Progenitor Cells
- Bioprinting of Collagen Hydrogels for Cartilage Substitutes
- Bioprinted Ears from an Integrated Tissue-?Organ Printer
- Decellularized Extracellular Matrix Bioink
- Decellularization: Re-?creating a Native Extracellular Matrix with Optimal Fidelity
- 4. Vascularization: Getting Blood from Here to There, Everywhere
- The Vasculature of the Human Body
- Blood Vessels Aren't Just Pipes
- Microchannel Networks Created with Fugitive Ink
- Multi-?nozzle Print Heads-?It's About Time
- Creating Vasculature in Bioprinted Tissues
- The Thickness Dilemma of Vascularized Tissue
- Responding to the Thickness Dilemma in Bioprinted Tissue
- The Splendid Scheme
- Perfused Vasculature Brings the Gift of Life
- Response to the Thickness Dilemma, Take Two
- 5. Innervation: The Body's Internet
- Nerve Growth Factor
- The Nervous System of the Human Body
- Bioprinting of a Cellular Nerve Guide
- Implanted Nerve Guides
- A Bioprinted Nerve Guide Using Growth Factors as Biochemical Cues
- Bioprinting Takes Aim at the Central Nervous System-?Spinal Cord Tissue
- Nervous System Nomenclature
- Optimizing Bioink for Induced Pluripotent Stem Cell-?Derived Neural Progenitor Cells
- Building Spinal Cord Scaffolds
- Cell Types Within Spinal Cord Scaffolds
- Calcium Imaging of Bioprinted Spinal Neurons
- 6. Skin and Bones-?and Muscle Too
- Give Me Some (Bioprinted) Skin
- Bioprinted Skin with Uniform Pigmentation
- Throw Me a (Bioprinted) Bone
- Morphing Cartilage to Bone: Emulating Embryonic Development
- A Succinct Sketch of Gene Therapy
- Gene Activated Bioinks
- Bioprinted Skeletal Muscle
- The Twitch
- Bioprinted Neuromuscular Junctions-?Inks from the Silkworm Bombyx mori
- Skeletal Muscle Alphabet Soup
- Cat's Cradle
- 7. The Liver: Regenerative Tissue That Can Almost Bioprint Itself
- The Hectic Life of the White Rabbit
- Cytoarchitecture
- Liver Cells Are Swimming in Blood
- Your Liver Is Like a Plush Bath Towel
- Bioprinting of Human Pluripotent Stem Cells for the Generation of Liver-?Like Cells
- Searching for Cell Markers
- Patterning of Cell-?Laden Hydrogels in the Form of Liver Tissue
- It's All Done with Mirrors
- Physiological Relevance
- Bioprinting Liver Tissue by Fusing Liver Bud Spheroids
- Caution: Road Work Ahead
- Human Liver Tissue Seeds
- A Historical Event: Organovo
- 8. The Heart: Cardiac Patches, Calcium, and Contraction Forces
- Keeping a Sense of Perspective
- The Sistine Chapel Ceiling Pre-?Michelangelo
- High-?Resolution Bioprinted Heart Patch
- Heart Attack
- Back from the Abyss
- Goosebump Moment
- Who Is to Blame for the Pipe Water?
- Pre-?Vascularized Bioprinted Heart Patch
- A Mini Noah's Ark
- Ejection Fraction and Echocardiograms
- Fractional Shortening
- Bioprinted Functional and Contractile Cardiac Tissue
- Hooke's Law
- Coming Back to Contractile Cardiac Tissue
- 9. Organs-?on-?a-?Chip: Tissues for Testing
- A Lilliputian Landscape
- Donald Ingber's Voyage to Organs-?on-?a-?Chip
- Tensegrity-?Say What?
- Geometric Control of Cell Life and Death
- Miniaturized Plumbing
- Bioprinted Three-?Tissue Organ-?on-?a-?Chip Platform
- Flexing Tissue Module Muscles One at a Time
- Flexing Tissue Module Muscles Two at a Time: Liver-? and Heart-?on-?a-?Chip Interactive Tests
- Lung Module Characterization
- Three at a Time: Liver, Heart, and Lung Interactive Tests-?Enter Sherlock Holmes
- The Plot Thickens
- Bioprinting a Functional Airway-?on-?a-?Chip
- Asthmatic Airway Epithelium on the Bioprinted Airway-?on-?a-?Chip
- Mucus
- Invasion of the Dust Mites
- Return Engagement
- Bioprinting Versus Manual Seeding
- Animal Testing
- 10. The Kidney: The Ne Plus Ultra of Filters
- The Nephron Is the Kidney's Workhorse
- Keeping on an Even Keel: Maintaining Electrolyte and Fluid Homeostasis
- Proximal Convoluted Tubules-?A Cherished Goal for Bioprinting
- Starting out on the Road to Proximal Convoluted Tubules on Perfusable Chips
- Printed Proximal Tubules Form a Polarized Epithelium
- Drug Toxicity Testing
- Vectorial Transport-?A Directed Passage
- Healthy and Mature Phenotypes
- Active, Selective Reabsorption of Key Solutes
- Measuring Albumin Reabsorption
- Measuring Glucose Reabsorption
- Hyperglycemia
- 11. What's in the Offing?
- A Thumbnail History of Polio
- Contemplating Bioprinting's Possible Future with a Sketch of Polio's History in Hand
- Vascularization and Scaling up to Human Size
- Aggregates of Pluripotent Stem Cells: Spheroids, Organoids, and Embryoid Bodies
- High Cellular Density with Vasculature via Embedded Printing
- A Slice May Suffice
- Organ Reserve
- A Portion Might Be the Prescription
- An Editorial Perspective
- Epilogue
- Notes
- Glossary
- Index
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