Essential Zebrafish Methods: Genetics and Genomics
Published on 5. September 2009
400 pages
978-0-12-375161-4 (ISBN)
Description
Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues that make it the model organism for study of vertebrate development.This book, one of two new volumes in the Reliable Lab Solutions series dealing with zebrafish, brings together a robust and up-to-date collection of time-tested methods presented by the world's leading scientists. Culled from previously published chapters in Methods in Cell Biology and updated by the original authors where relevant, it provides a comprehensive collection of protocols describing the most widely used techniques relevant to the study of zebrafish genetics and genomics. The methods in this volume were hand-selected by the editors, whose goal was to a provide a handy and cost-effective collection of fail-safe methods, tips, and "tricks of the trade" to both experienced researchers and more junior members in the lab.
- Provides busy researchers a quick reference for time-tested methods and protocols that really work, updated where possible by the original authors
- Gives pragmatic wisdom to the non-specialist from experts in the field with years of experience with trial and error
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H. William Detrich III | Leonard I. Zon | Monte Westerfield
Essential Zebrafish Methods: Genetics and Genomics
Book
10/2009
Academic Press
€71.19
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Content
1. Haploid Screens and Gamma-Ray Mutagenesis Charline Walker2. Positional Cloning of Mutated Zebrafish Genes Will Talbot3. The Zebrafish Genome John Postlethwait4. Retroviral-Mediated Insertional Mutogenesis in Zebrafish Adam Amsterdam5. Genetic Screens for Maternal-Effect Mutations Francisco Pelegri6. Behavior Screening Assays in Zebrafish Herwig Baier7. A High-Throughput Method for Identifying N-Ethyl-N-Nitrosourea (ENU)-Induced Point Mutations in Zebrafish Cecelia B. Moens8. Transgenesis and Gene Trap Methods in Zebrafish by Using the Tol2 Transposable Element Koichi Kawakami9. Molecular Cytogenetic Methodologies and a Bacterial Artificial Chromosome (BAC) Probe Panel Resource for Genomic Analysis in Zebrafish Charles Lee10. The Zon Laboratory Guide to Positional Cloning in Zebrafish Leonard I. Zon11. Sleeping Beauty Transposon for Efficient Gene Delivery Stephen C. Ecker12. Transgene Manipulation in Zebrafish by Using Recombinases Gary W. Stuart13. Highly Efficient Zebrafish Transgenesis Mediated by the Meganuclease I-SceI Joachim Wittbrodt14. Cloning Zebrafish by Nuclear Transfer Shuo Lin15. Spatial and Temporal Expression of the Zebrafish Genome by Large-Scale In Situ Hybridization Screening Christine Thisse16. Genetic Backgrounds, Standard Lines, and Husbandry of Zebrafish Bill Trevarrow17. Common Diseases of Laboratory Zebrafish Jennifer L. Matthews18. Zebrafish Sperm Cryopreservation Thomas Look
