Current Topics in Membranes and Transport
Molecular approaches to epethelial transport
Academic Press
Published on 1. February 1984
307 pages
978-0-08-058491-1 (ISBN)
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Current Topics in Membranes and Transport
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Felix Bronner | A. Kleinzeller
Current Topics in Membranes and Transport: Molecular Approaches to Epithelial Transport v. 20
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02/1984
Academic Press
€69.33
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Content
- Front Cover
- Molecular Approaches to Epithelial Transport
- Copyright Page
- Contents
- Contributors
- Preface
- Yale Membrane Transport Processes Volumes
- Part I: Frequency Domain Analysis of Ion Transport
- Chapter 1. Fluctuation Analysis of Apical Sodium Transport
- I. Introduction
- II. Mechanics of Fluctuation Analysis
- III. Epithelial Sources of Fluctuations
- IV. Fluctuations in Apical Sodium Conductance
- V. Model Predictions
- VI. Conclusion
- References
- Chapter 2. Impedance Analysis of Necturus Gallbladder Epithelium Using Extra- and Intracellular Microelectrodes
- I. Introduction
- II. Data Analysis Technique
- III. Equivalent-Circuit Models of Necturus Gallbladder Epithelium
- IV. Transepithelial and Apparent Intracellular Transfer Functions under Control Conditions
- V. Impedance Loci under Current-Induced Changes of Lateral Space Width
- VI. Conclusions
- References
- Chapter 3. Membrane Area Changes Associated with Proton Secretion in Turtle Urinary Bladder Studied Using Impedance Analysis Techniques
- I. Introduction
- II. Methods
- III. Results
- IV. Discussion
- References
- Chapter 4. Mechanisms of Ion Transport by the Mammalian Colon Revealed by Frequency Domain Analysis Techniques
- I. Overview of the Basic Features of the Rabbit Descending Colon
- II. Impedance Analysis
- III. Current Fluctuation Analysis
- IV. Summary
- References
- Chapter 5. Analysis of Ion Transport Using Frequency Domain Measurements
- I. Introduction
- II. Black Box Analysis of Epithelial Transport
- III. Dissociation of Membrane Properties
- IV. Questions for Analysis in the Frequency Domain
- V. Methods for Frequency Analysis
- VI. Impedance Analysis
- VII. Fluctuation Analysis
- VIII. Summary
- References
- Chapter 6. Use of Potassium Depolarization to Study Apical Transport Properties in Eplthelia
- I. Introduction
- II. Uses of K+ Depolarization
- III. Basis for the K +-Depolarization Technique
- IV. Evaluation of Depolarization
- V. Conclusions
- References
- Part II: Use of Antibodies to Epithelial Membrane Proteins
- Chapter 7. Biosynthesis of Na+ ,K+ -ATPase in Amphibian Epithelial Cells
- I. Introduction and General Background
- II. Purification of Na +, K + -ATPase
- III. Preparation and Characterization of Immunological Probes
- IV. Biosynthesis of Na+ ,K + -ATPase in Amphibian Epithelial Cells
- V. Hormonal Control of Na + ,K +-ATPase Synthesis in Amphibian Epithelial Cells
- VI. Possible Sites of Action of Hormones on the Control of Synthesis and Expression of Na + ,K +-ATPase: A Model
- VII. Concluding Remarks
- References
- Chapter 8. Use of Antibodies in the Study of Na+,K+-ATPase Biosynthesis and Structure
- I. Introduction
- II. Methodology: Immune Precipitation and Solid Phase Assay
- III. Applications: Biosynthesis and Structure
- IV. Problems Associated with Antibody Detection of Membrane Proteins
- References
- Chapter 9. Encounters with Monoclonal Antibodies to Na+,K+-ATPase
- I. Introduction
- II. Immunization Procedures
- III. Screening Procedures
- IV. Generation, Purification, and Characterization of Monoclonal Immunoglobulin
- V. Morphologic Application of Monoclonal Antibodies to Membrane Surface Proteins
- VI. Summary
- References
- Chapter 10. Monoclonal Antibodies as Probes of Epithelial Cell Polarity
- I. Production of Monoclonal Antibodies against MDCK Cell Surface Proteins
- II. Distribution of Polarized Cell Surface Proteins
- III. Development and Maintenance of Epithelial Cell Polarity
- IV. Demonstration of Nephron Segment-Specific Proteins
- References
- Chapter 11. Immunolabeling of Frozen Thin Sections and Its Application to the Study of the Biogenesis of Epithelial Cell Plasma Membranes
- I. Introduction
- II. Production of Ultrathin Frozen Sections
- III. Indirect Immunolabeling
- IV. Embedding and Staining of Frozen Thin Sections
- V. Concluding Remarks
- References
- Chapter 12. Development of Antibodies to Apical Membrane Constituents Associated with the Action of Vasopressin
- I. Introduction
- II. Vasopressin Action
- III. Antibody Assay Strategy
- IV. Characterization of Antibodies
- V. Concluding Remarks
- References
- Chapter 13. Molecular Modification of Renal Brush Border Maltase with Age: Monoclonal Antibody-Specific Forms of the Enzyme
- I. Introduction
- II. Purification of Maltase
- III. Production of Antibodies to "Young" and "Old" Enzyme
- IV. Characterization of Antibodies
- V. Conclusions
- References
- Part III: Biochemical Characterization of Transport Proteins
- Chapter 14. Sodium-D-Glucose Cotransport System: Biochemical Analysis of Active Sites
- I. Introduction
- II. The Possible Polymeric Structure of the Sodium-D-Glucose Cotransport System
- III. Characterization of the Functionally Active Sites
- IV. Summary and Conclusions
- References
- Chapter 15. Probing Molecular Characteristics of Ion Transport Proteins
- I. Introduction
- II. A Phosphate-Binding Proteolipid
- III. Functional Residues of the Na+ Channel
- IV. How Does Amiloride Inhibit the Na+ Channel?
- V. Summary
- References
- Chapter 16. Aldosterone-lnduced Proteins in Renal Epithelia
- I. Introduction
- II. Subcellular Site of Action of Aldosterone
- III. Aldosterone-lnduced Proteins: Detection
- IV. Aldosterone-lnduced Proteins: Characterization by Two-Dimensional Polyacrylamide Gel Electrophoresis
- V. Summary
- References
- Chapter 17. Development of an Isolation Procedure for Brush Border Membrane of an Electrically Tight Epithelium: Rabbit Distal Colon
- I. The Need to Isolate Brush Border Membrane from Tight Epithelia
- II. Approach to Problems of Brush Border Membrane Isolation in Rabbit Descending Colon
- III. Ouabain-Insensitive K + -Dependent Phosphohydrolase
- IV. Critical Evaluation of Isolation Technique-Future Utility
- References
- Index
- Contents of Previous Volumes
