Techniques in Confocal Microscopy

Part I. Practical Considerations and Equipment1. Theoretical Basis of Confocal MicroscopyRobert H. Webb2. Practical Considerations in Acquiring Biological Signals from Confocal MicroscopeHao He, Rose Chik Ying Ong, Kam Tai Chan, Ho Pui Ho, Siu Kai Kong3. Equipment for Mass Storage and Processing of DataGuy Cox4. Antifading Agents for Confocal Fluorescence MicroscopyMiguel Berrios, Kimberly A. Conlon, and David E. Colflesh5. Mounting Techniques for Confocal MicroscopyManabu Kagayama and Yasuyuki Sasano6. Preparation of Whole Mounts and Thick Sections for Confocal MicroscopyHisashi Hashimoto, Hiroshi Ishikawa, and Moriaki Kusakabe7. Use of Confocal Microscopy to Investigate Cell Structure and FunctionGhassan Bkaily, Danielle Jacques8. Combining Laser Scanning Confocal Microscopy and Electron MicroscopyXuejun SunPart II. Functional Approaches9. Volume Measurements in Confocal MicroscopyCarlos B. Mantilla, Y.S. Prakash and Gary C. Sieck10. Quantitation of Phagocytosis by Confocal MicroscopyGeorge F. Babcock and Chad T Robinson MS11. Receptor-Ligand InternalizationGuido Orlandini, Nicoletta Ronda, Rita Gatti, Gian Carlo Gazzola, and Alberico Borghetti12. Quantitative Imaging of Metabolism by Two-Photon Excitation MicroscopyDavid W. Piston and Susan M. Knobel13. Trafficking of the Androgen ReceptorVirginie Georget, Béatrice Terouanne, Jean-Claude Nicolas, and Charles Sultan14. Localization of Proteases and Peptide Receptors by Confocal MicroscopyKanti D. Bhoola, Celia J. Snyman and Carlos D. Figueroa15. Multiphoton Excitation Microscopy, Confocal Microscopy, and Spectroscopy of Living Cells and Tissues: Functional Metabolic Imaging of Human Skin in VivoBarry R. Masters, Peter T. C. So, Ki Hean Kim, Christof Buehler, and Enrico Gratton16. Video-Rate, Scannng Slit Confocal Microscopy of Living Human Cornea in Vivo: Three-Dimensional Confocal Microscopy of the EyeBarry R. Masters and Matthias Böhnke17. In Vivo Imaging of Mammalian Central Nervous System Neurons with the In Vivo Confocal Neuroimaging (ICON) MethodSylvia Prilloff, Petra Henrich-Noack, Ralf Engelmann, Bernhard A. Sabel18. Identification of Viral Infection by Confocal MicroscopyDavid N. Howell and Sara E. Miller19. Membrane TraffickingSabine Kupzig, San San Lee, and George BantingPart III. Green Fluorescent Protein20. Monitoring of Protein Secretion with Green Fluorescent ProteinChristoph Kaether and Hans-Hermann Gerdes21. Comparison of Enhanced Green Fluorescent Protein and Its Destabilized Form as Transcription ReportersXiaoning Zhao, Tommy Duong, Chiao-Chian Huang, Steven R. Kain, and Xianqiang Li22. Measuring Protein Degradation with Green Fluorescent ProteinStephen R. Cronin and Randolph Y. Hampton23. Studying Nuclear Receptors with Green Fluorescent Protein FusionsGordon L. Hager24. Signaling, Desensitization, and Trafficking of G Protein-Coupled Receptors Revealed by Green Fluorescent Protein ConjugatesLarry S. Barak, Jie Zhang, Stephen S. G. Ferguson, Stephane A. Laporte, and Marc G. Caron25. Fluorescent Proteins in Single- and Multicolor Flow CytometryLonnie Lybarger and Robert Chervenak26. Jellyfish Green Fluorescent Protein: A Tool for Studying Ion Channels and Second-Messenger Signaling in NeuronsL. A. C. Blair, K. K.Bence, and J. Marshall27. Expression of Green Fluorescent Protein and Inositol 1,4,5-Triphosphate Receptor in Xenopus laevis OocytesAtshushi Miyawaki, Julie M. Matheson, Lee G. Sayers, Akira Muto, Takayuki Michikawa, Teiichi Furuichi, and Katsuhiko Mikoshiba28. Confocal Imaging of Ca2+, pH, Electrical Potential, and Membrane Permeability in Single Living CellsJohn J. Lemasters, Donna R. Trollinger, Ting Qian, Wayne E. Cascio, and Hisayuki OhataPart IV. Laser Capture Microdissection29. Laser Capture Microdissection and Its Applications in Genomics and ProteomicsJames L. Wittliff30. Going in Vivo with Laser MicrodissectionAnette Mayer, Monika Stich, Dieter Brocksch, Karin Schütze, and Georgia Lahr31.