Alles über E-Books | Antworten auf Fragen rund um E-Books, Kopierschutz und Dateiformate finden Sie in unserem Info- & Hilfebereich.
Methods in Neurosciences, Volume 9: Gene Expression in Neural Tissues describes methods for the study of gene expression in neural tissues. Cloning, gene transfer, and Northern blotting for quantifying rare messenger RNAs are considered. The discussions are organized around five themes: expression of specific genes; in situ hybridization; gene transfer; regulation of expression; and general approaches. Comprised of 30 chapters, this volume first examines calbindin D28K gene expression in neurodegenerative diseases such as Parkinson's disease, Huntington's disease, and Alzheimer's disease, and the role of calbindin D28K in the etiology and pathogenesis of neurodegeneration in the brain. The reader is then introduced to gene expression of corticotropin-releasing factor, dystrophin, cytokines, and neurotrophic factors. Subsequent chapters focus on the gene expression of rat brain/Hep G2 glucose transporter, insulin-like growth factor II, and neuropeptide Y as well as proopiomelanocortin and neurotrophin receptors. The book also discusses gene regulation analysis by lipopolyamine-mediated DNA transfer in primary neurons; characterization of neuron-specific transcription factors in Drosophila melanogaster; and cloning of dopamine receptors using a homology approach. This monograph will be of interest to students and practitioners in the fields of molecular genetics, microbiology, neuroendocrinology, neurology, and other branches of the neurosciences.
Language
Place of publication
File size
ISBN-13
978-1-4832-5965-9 (9781483259659)
Schweitzer Classification
¿Contributors to Volume 9PrefaceVolumes in SeriesSection I Expression of Specific Genes 1. Calbindin D28K Gene Expression in Neurodegenerative Diseases 2. Corticotropin-Releasing Factor Gene Expression 3. Gene Expression of Cytokines and Neurotrophic Factors in Human Brain Tumors 4. Dystrophin Gene Expression in Neuronal Tissues 5. Neuropeptide FMRFamide Gene Expression 6. Rat Brain/Hep G2 Glucose Transporter Gene Expression in Brain 7. Insulin-Like Growth Factor II Gene Expression 8. Expression of Mouse Interphotoreceptor Retinoid-Binding Protein Gene during Development 9. Analysis of Muscarinic Acetylcholine Receptor Expression and Function 10. Expression of Human Neuropeptide Y Gene in Neuronal Cell Lines 11. Neuropeptide Y Gene Expression 12. Neurotrophin Receptor Gene Expression 13. Detection of Nerve Growth Factor and One of Its Receptors 14. Assessment of Proopiomelanocortin Gene Expression in Brain 15. Weaver Gene Expression in Central Nervous SystemSection II In Situ Hybridization 16. In Situ Hybridization and Immunohistochemical Methods in Study of Regulatory Molecules 17. Using mRNA in Situ Hybridization to Localize Wnt-3 and Wnt-3A Expression in Developing Neural Tube 18. In Situ Hybridization for Neurofilament and GAP-43 mRNA in Primary Sensory Neurons in Conjunction with Nerve Growth Factor Receptor RadioautographySection III Gene Transfer 19. Gene Regulation Analysis by Lipopolyamine-Mediated DNA Transfer in Primary Neurons 20. Retro virus-Mediated Gene Transfer into Mouse Cerebellar Cultures 21. Analysis of Cis-Acting Elements of Oxytocin Gene by DNA-Mediated Gene TransferSection IV Regulation of Expression 22. Identification of Cis-Regulatory Sequences Required for Induction of Neurotensin/Neuromedin N Gene Expression in PC12 Cells 23. Characterization of Neuron-Specific Transcription Factors in Drosophila melanogaster 24. DNA-Protein Interactions in Phenotypically Identified Neurons in Discrete Regions of Intact BrainSection V General Approaches 25. Enhancer Trap Method in Drosophila: Its Application to Neurobiology 26. Use of Northern Blotting for Quantifying Rare mRNAs in Neurons of Developing Peripheral Nervous System 27. Dopamine D2 Receptors: Isolation of Cell Lines Using Ligand Binding 28. Cloning of Dopamine Receptors: Homology Approach 29. Cloning of Human Thyrotropin-Releasing Hormone cDNA Using Degenerate Oligonucleotide Probes and Tetramethylammonium Chloride 30. In Vivo Analysis of Gene Expression in Central Catecholamine CellsIndex