Essential Ion Channel Methods

PART I. Assembly1. Assembly of Ion ChannelsZuFang Sheng and Carol Deutsch PART II. Genetics2. Identification of Ion Channel-Associated Proteins Using the Yeast Two-Hybrid SystemMartin Niethammer and Morgan Sheng PART III. Electrophysiology3. Patch Clamp Studies of the Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channel John W. Hanrahan, Zie Kone , Ceri J. Mathews,Jiexin Luo, Yanlin Jia and Paul Lindsell4. Tight-Seal Whole-cell Patch Clamping of C. elegans NeuronsS.R. Lockery and M.B. Goodman5. Gating Currents Enrico Stefani and Francisco Bezanilla6. Determining Ion Channel Permeation PropertiesTed Begenisich PART IV. Expression Systems7. Expression of Ligand-Gated Ion Channels Using Using Semliki Forest Virus and BaculovirusKathryn Radford and Gary Buell8. Recombinant Adenovirus-Mediated Expression in Nervous System of Genes Coding for Ion Channels and Other Molecules Involved in Synaptic FunctionMarkus U. Ehrengruber, Markus Lanzrein, Youfeng Xu, Mark C. Jasek, David B. Kantor, Erin M. Schuman, Henry A. Lester, and Norman Davidson9. Transient Expression of Heteromeric Ion ChannelsAlison L. Eertmoed, Yolanda F. Vallejo, and William N. Green PART V. Model Simulations10. Computer Simulations and Modeling of Ion ChannelsMichael E. Green PART VI. Physical11. Fluorescence Techniques for Measuring Ion Channel ActivityGönül Velicelebi, Kenneth A. Stauderman, Mark A. Varney, Michael Akong, Stephen D. Hess, and Edwin C. Johnson12. Ligand Binding Methods for Analysis of Ion Channel Structure and FunctionSteen E. Pedersen, Monica M. Lurtz, and Rao V. L. Papineni13. Three-dimensional structure of membrane proteins determined by two-dimensional crystallization, electron cryomicroscopy, and image analysisMark Yeager, Vinzenz M. Unger, Alok K. Mitra14. Voltage Clamp Biosensors for Capillary ElectrophoresisOwe Orwar, Kent Jardemark, Cecilia Farre, Ingemar Jacobson, Alexander Moscho, Jason B. Shear, Harvey A. Fishman, Sheri J. Lillard, Richard N. Zare15. Ion Channels As Tools to Monitor Lipid Bilayer-Membrane Protein Interactions: Gramicidin Channels As Molecular ForceO. S. Andersen, C. Nielsen, A. M. Maer, J. A. Lundbæk, M. Goulian, and R. E. Koeppe I PART VII. Purification and Reconstitution16. Purification and Reconstitution of the Epithelial Chloride Channel Cystic Fibrosis Transmembrane Conductance RegulatorMohabir Ramjeesingh, Elizabeth Garani, Kevin Galley, Canhui Li, Yanchun Wang, Christine E. Bear17. Reconstitution of Native and Cloned Channels into Planar BilayersI. Favre, Y.-M. Sun, and E. Moczydlowski PART VIII. Second Messengers and Biochemical Approaches18. Protein Phosphorylation of Ligand-Gated Ion ChannelsAndrew l. Mammen, Sunjeev Kamboj, and Richard L. Huganir19. Analysis of Ion Channel Associated ProteinsMichael Wyszynski and Morgan Sheng20. Secondary Messenger Regulation of Ion Channels/Plant Patch ClampingSarah M. Assmann and Lisa Romano PART IX. Special Channels21. ATP-Sensitive Potassium ChannelsJoseph Bryan, M. Schwanstecher, C. Schwanstecher, F. Chudziak, U. Panten, J.P. Clement IV, G. Gonzalez, and L. Aguilar-Bryan22. Simplified Fast Pressure-Clamp Technique for Studying Mechanically-Gated ChannelsDon W. McBride, Jr. and Owen P. Hamill23. Purification and Heterologous Expression of Inhibitory Glycine ReceptorsBodo Laube and Heinrich Betz24. Functional Analyses of Aquaporin Water Channel ProteinsPeter Agre, John C. Mathai, Barbara L. Smith, and Gregory M. Preston PART X. Toxins and Other Membrane Active Compounds25. Conus Peptides as Probes for Ion ChannelsJ. Michael McIntosh, Baldomero M. Olivera, and Lourdes J. Cruz26. Scorpion Toxins as Tools for Studying Potassium ChannelsMaria Garcia, Markus Hanner, Hans-Günther Knaus, Robert Slaughter, Gregory J. Kaczorowski27. The Use of Planar Lipid Bilayer Membranes for Rapid Screening of Membrane Active CompoundsBruce L. Kagan, Tajb A. Mirzabekov, Antoly Y. Silberstein28. Antibodies to Ion ChannelsAngela Vincent, Ian Hart, Ashwin Pinto, and F.