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Gene Expression provides research papers on selected topics in gene expression, presented at the 11th meeting of the Federation of European Biochemical Societies, held at Copenhagen in August 1977. The book presents research knowledge provided by eminent researchers in the field of biochemistry. Each chapter contains material that is important to other researchers, such as on initiation mechanism of protein synthesis in prokaryotes; translocation mechanism of the ribosome; and analysis of ribosomal translocation by drugs. Mechanisms for the intracellular compartmentation of newly synthesized proteins; RNA synthesis and control; the sub-structure of nucleosome core particles; and future prospects on chromosome structure and function are detailed as well. The text will be of use to researchers and workers in the field of medicine, pharmacology, gene therapy, and biochemistry.
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978-1-4831-5406-0 (9781483154060)
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General Introduction to the ProceedingsForewordSection A. Protein Synthesis and Control Introduction 1. Initiation and Control of Protein Synthesis Initiation Mechanism of Protein Synthesis in Prokaryotes Eukaryotic Initiation of Protein Synthesis Regulation of Eukaryotic Protein Chain Initiation Cryptic Initiation Sites in Eukaryotic Virus mRNAs Interferon-induced Translational Regulation 2. Elongation and Control of Protein Synthesis The Binding of AAtRNA to Prokaryotic Ribosomes Trans-location Mechanism of the Ribosome Analysis of Ribosomal Translocation by Drugs Regulation of Esoheriohia ooli Elongation Factor. Synthesis In Vivo Mechanisms for the Intracellular Compartmentation of Newly Synthesized Proteins 3. Structure and Function of Components of Protein Synthesis Ribosomal Protein-Nucleic Acid Interaction Ribosome Structure and Functional SitesSection B. RNA Synthesis and Control Introduction Binding of E. Coli RNA Polymerase to Poly (d(A-s4T)) Studies Using the Filter Retention Assay Control of E. Coli rDNA Transcription In Vivo and In Vitro RNA Synthesis Termination Factor Rho The Role of Rho in Transcription Termination in E. Coli Biochemistry of Development of E. Coli Viruses T7 and T1 Eukaryotic RNA Polymerases In Vitro Transcription of the Adenovirus Type 2 GenomeSection C. Chromatin Structure and Function Introduction The Sub-Structure of Nucleosome Core Particles Studies on Chromatin Structure by Nuclease Digestion X-Ray Crystallographic and Enzymatic Analysis of Nucleosome Cores Higher Order Structure of Mitotic Chromosomes Isolation and Characterization of Eukaryotic DNA Fragments Containing Structural Genes and Adjacent Sequences Structure and Transcription of the Free Ribosomal RNA Genes in Tetrahymena Suppression of Transcription in Balbiani Ring 2 and the Effect on Chromosome Structure The Effect of Heat Shock on Gene Expression in Drosophila Melanogaster Chromosome Structure and Function: Future ProspectsIndex