Probes and Membrane Function
Published on 2. October 2013
582 pages
978-1-4832-7148-4 (ISBN)
Description
Probes of Structure and Function of Macromolecules and Membranes, Volume I: Probes and Membrane Function discusses the developments in techniques for the measurement of rapid reactions and detection of enzymatic reactions in simple and complex systems. This book describes the application of the incisive techniques employed for the study of activity-related structural changes in enzymes to functionally related structural changes in membranes. The structural changes initiated in response to energy coupling and ion movements in mitochondria and axons are also elaborated. This publication covers the basic mechanisms by which structural changes can participate in energy coupling at the redox level of the hemoproteins that leads to the evaluation of the function of ubiquinone and cytochrome b in energy coupling. The function of factors that couple electron transport and energy conservation is likewise considered. This volume is a good reference for students and researchers conducting work on the structure and function of macromolecules and membranes.
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Probes of Structure and Function of Macromolecules and Membranes: Probes and Membrane Function v. 1
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12/1971
Academic Press
€85.42
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Content
ContributorsPrefaceContents of Volume IIIntroductory RemarksGeneral Introduction to Responsive ProbesPart 1. Interpretation of Data from Probe Studies Introduction to Fluorescence Probes The Effects of Chemical Environment on Fluorescence Probes Discussion The Use of Specific Chromophoric Inhibitors in Investigations of Biological Reactions in Solution, in Crystals, and in Membranes Structural Sensitive Aspects of the Electronic Spectrum Discussion Introduction to Magnetic Resonance Techniques The Nuclear Magnetic Resonance Spectroscopy of Proteins Paramagnetic Probes in Enzyme Systems Enzyme-Ligand Interactions as Detected by Nuclear Relaxation Rates of Substrates and Inhibitors Electron-Nuclear Double Resonance (ENDOR) from Randomly Oriented Biomolecules Electron Paramagnetic Resonance of Single Crystals as Environmental Probes Discussion Optical Activity and Conformation of Proteins Near Infrared Circular Dichroism: d ¿ d Transitions in Hemoproteins Discussion Introduction to X-Ray Diffraction and Electron Microscopy Studies Protein Conformational Changes Studied by Diffraction Techniques Low-Angle X-Ray Diffraction of Biological Membranes Probing Membrane Structure with the Electron Microscope Characterization of a Hydrocarbon Soluble Protein/Lipid Complex DiscussionPart 2. Structural Control of Reactivity of Membranes State Changes of the Mitochondrial Membrane as Detected by the Fluorescence Probe 1-Anilino-8-naphthalene Sulfonic Acid Probes of Macromolecular and Molecular Structure in the Membranes of Mitochondria and Submitochondrial Vesiqles Fluorochrome Labeling of Chromatophores of Photosynthetic Bacteria with Auramine O Fluorescence Changes in Dye-Treated Nerve following Electric Stimulation Anilino-1, 8-naphthalene Sulfonate and Brom thymol Blue Responses to Membrane Energization Kinetic Analysis of MNS Interaction with Submitochondrial Particles Discussion Spin Labeling of Submitochondrial Membrane Discussion Response of ANS to Ca++ Binding in Mitochondrial Membrane as Affected by Local Anesthetics K+ Gradients in Submitochondrial Particles K+ Transport in Submitochondrial Particles Glutaraldehyde as a Probe of Metabolism-Linked Changes in the Mitochondrial Proteins Calcium Transport in Sonicated Submitochondrial Particles General Discussion: Responses of Probes to Ionic Gradients Use of 8-Anilino-1-naphthalene Sulfonate as Conformational Probe on Biological Membranes ANS Fluorescence as an Indicator of Ionic Interaction with Membranes Interaction of Fluorescent Probes with Hemoglobin Free Erythrocyte Membranes The Design of Fluorescent Probes for Membranes Conformational Activity vs.
