Cellular Immunology LabFax

¿ContributorsAbbreviations1. Laboratory Animals Welfare of Laboratory Animals Health Monitoring Anesthesia and Analgesia Pre-Anesthesia Anesthesia Analgesia Animal Models in Immunology Induced Animal Models Spontaneous Animal Models Transplantation Immunology Graft versus Host Reaction Immunization of Animals for Antibody Production Polyclonal Antibody Production Monoclonal Antibody Production Antigen Purity and Dose Adjuvants Routes of Administration A Standard Procedure for the Production of Monoclonal Antibodies A Standard Procedure for the Production of Polyclonal Antibodies Bleeding References2. Tissues and Cells of the Immune System Primary Lymphoid Tissues Thymus Bursa of Fabricius Mammalian B Lymphopoiesis Secondary (Peripheral) Lymphoid Tissues Lymph Nodes Spleen Mucosa-Associated Lymphoid Tissue (MALT) Lymphocyte Recirculation and Homing Cells of the Immune Response Lymphocytes Nonlymphoid Cells References3. Leukocyte Development Hematopoiesis Lymphocyte Development B Cells T Cells Markers of Cellular Activation Leukocyte Lifespans Age Dependence of Lymphocyte Subpopulations Methods of Studying Lymphocyte Development References4. Tissue and Cell Culture General Information Biosafety Cabinets Other Required Equipment Methods of Sterilization Biosafety Levels Tissue Culture Media Media Formulations Supplements and Media Modifications Antibiotics Organ Culture Long-Term Bone Marrow Cultures Thymic Organ Cultures Splenic Fragment Cultures Cell Isolation Methods of Tissue Dissociation Enumeration of Cell Number Washing and Removal of Dead Cells Cell Separation Techniques Dyes Used in FACS Analysis and Cell Sorting Cell Culture Cell Density Use of Feeder Cells Inactivation of Cells/Cell Substitutes Use of Culture Supernatants Polyclonal Activators Cell and Tissue Preservation Fixation Cryopreservation References5. Cell Lines and Hybridomas Cell Lines Viral- and Gene-Mediated Transformation Stromal Cell-Dependent B-Cell and T-Cell Lines Antigen-Specific T-Cell Lines Murine Helper T-Cell Lines Human Helper T-Cell Lines B-Cell Hybridomas General Aspects of Hybridoma Production Myelomas Normal Lymphocytes Cell Fusion Selection of Hybridomas Screening Culture of Hybridoma Clones Application of Monoclonal Antibodies T-Cell Hybridomas T-Lymphoma Lines Normal T Lymphocytes Fusion and Selection Screening Cloning Definition and Purpose Procedures Verification of Clonality Cell-Line Suppliers References6. Assays of Cellular Activity Measurement of Early Activation Phagocytosis and Microbicidal Activity Chemotaxis and Chemokinesis Proliferation Cytokine Production Antibody Secretion Cytotoxicity Helper Function TH Lymphokine Secretion Supernatant Helper Function Limiting Dilution Apoptosis References7. Cell-Surface Antigens Introduction References8.