Cell Biology

Contents of Volume 1Part A. Cell and Tissue Culture: Associated Techniques.Section 1. General Techniques.Section 2. Culture of Specific Cell Types: Stem Cells.Section 3. Culture of Specific Cell Types: Haemopoietic, Mesenchymal, and Epithelial.Section 4. Differentiation and Reprogramming of Somatic Cells.Section 5. Immortalisation.Section 6. Somatic Cell Hybrids.Section 7. Cell Separation Techniques.Section 8. Cell Cycle Analysis.Section 9. Cytotoxic and Cell Growth Assays.Section 10. Apoptosis.Section 11. Assays of Cell Transformation, Tumorigenesis, Invasion and Wound Healing.Section 12. Electrophysiological Methods.Section 13. Organ Cultures.Part B. Viruses.Section 14. Growth and Purification of Viruses.Part C. Antibodies.Section 15. Production and Purification of Antibodies.Part D. Immunocytochemistry.Section 16. Immunofluoresence.Part E. Appendix. Contents of Volume 2Part A. Organelles and Cellular Structures.Section 1. Isolation: Plasma Membrane, Organelles, and Cellular Structures.Section 2. Vital Staining of Cells/Organelles.Section 3. Protein Purification.Part B. Assays.Section 4. Endocytic and Exocytic Pathways.Section 5. Membranes.Section 6. Mitochondria.Section 7. Nuclear Transport.Section 8. Chromatin Assembly.Section 9. Signal Transduction Assays.Section 10. Assays and Models of in Vitro and in Vitro Motility.Section 11. Mechanical Stress in Single Cells.Part C. Appendix. Contents of Volume 3Part A. Imaging Techniques.Section 1. Light Microscopy.Section 2. Digital Video Microscopy.Section 3. Confocal Microscopy of Living Cells and Fixed Cells.Section 4. Fluorescent Microscopy of Living Cells.Section 5. Use of Fluorescent Dyes for Studies of Intracellular Physiological Parameters.Section 6. Digital Image Processing, Analysis, Storage, and Display.Part B. Electron Microscopy.Section 7. Specimen Preparation Techniques.Section 8. Cryotechniques.Section 9. Electron Microscopy Studies of the Cytoskeleton.Section 10. Immunoelectron Microscopy.Part C. Scanning Probe and Scanning Electron Microscopy.Section 11. Scanning Probe and Scanning Electron Microscopy.Part D. Microdissection.Section 12. Tissue and Chromosome Microdissection.Part E. Tissue Arrays.Section 13. Tissue Arrays.Part F. Cytogenetics and in Situ HybridisationSection 14. Cytogenetics.Section 15. In Situ Hybridisation.Part G. Genomics.Section 16. Genomics.Part H. Transgenic, Knockouts, and Knockdown Methods.Section 17. Transgenic, Knockouts and knock-down methods. Contents of Volume 4Part A. Transfer of Macromolecules.Section 1. Proteins.Section 2. Genes.Section 3. Somatic Cell Nuclear Transfer.Part B. Expression Systems.Section 4. Expression Systems.Part C. Gene Expression Profiling.Section 5. Differential Gene Expression.Part D. Proteins.Section 6. Protein Determination and Analysis.Section 7. Sample Fractionation for Proteomics.Section 8. gel Electrophoresis.Section 9. Detection of Proteins in Gels.Section 10. Gel Profiling of Posttranslationally Modified Proteins.Section 11. Protein/Protein and Protein/Small Molecule Interactions.Section 12. Functional Proteomics.Section 13. Protein/DNA Interactions.Section 14. Protein Degradation.Section 15. Mass Spectrometry: Protein Identification and Interactions.Part E. Appendix.