Biochemical Applications of Raman and Resonance Raman Spectroscopes
Published on 2. December 2012
276 pages
978-0-323-15812-1 (ISBN)
Description
Biochemical Applications of Raman and Resonance Raman Spectroscopies focuses on the application of Raman and resonance Raman spectroscopies to biochemical problems. The book reviews biological systems and details the application of Raman spectroscopy to biological molecules such as proteins, nucleic acids, and lipids. It also looks at codevelopments of lasers, optics, and electronics that drive advances in experimental Raman spectroscopy, along with the important ramifications of these advances for biochemical applications. This volume is organized into eight chapters and begins with an overview of the theoretical and experimental aspects of Raman spectroscopy, including a very brief explanation of what Raman and resonance Raman spectroscopies are and a discussion of their advantages and disadvantages for biochemical studies. The explanation of the Raman and resonance Raman effects is taken up in more detail in the next chapter, which develops the concept of the vibrational motions of molecules by initially considering mechanical ""ball and spring"" models and goes on to use this concept to formulate a classical model for Raman scattering. The resonance Raman effect is then described by another model which emphasizes the discrete or quantized energy levels available to a molecule. The reader is also introduced to the experimental aspects of Raman spectroscopy and the application of Raman spectroscopy across the entire field of biochemistry. Each chapter contains an outline of the basic chemistry and biochemical nomenclature involved. This book will be of interest to chemists, biochemists, and spectroscopists, as well as graduate students and experienced research workers.
More details
Other editions
Additional editions
Book
01/1982
Academic Press
€94.71
Article exhausted; check different version
Content
PrefaceChapter l. Introduction I. Raman Scattering II. The Appearance of a Raman Spectrum III. Basic Units IV. Raman, Resonance Raman, and Infrared Spectroscopies V. Advantages and Disadvantages of Raman Spectroscopy for Biochemical StudiesChapter 2. Principles of Raman Spectroscopy I. Introduction II. The Vibrations of a Diatomic Molecule from a Classical Standpoint III. A Classical Model for Raman Scattering IV. Comparison with Infrared Spectroscopy V. A Quantum Mechanical Picture of Raman Scattering VI. The Vibrations of a Polyatomic Molecule VII. Polarization Properties of Raman Scattering VIII. Raman IntensitiesChapter 3. Experimental Raman Spectroscopy I. Basic Optics of the Raman Experiment II. Sampling Techniques and Sample Problems III. Sophisticated TechniquesChapter 4. Protein Conformation from Raman and Resonance Raman Spectra I. Protein Structure II. Introduction to Raman and Resonance Raman Studies of Proteins III. Raman Studies of Proteins IV. Resonance Raman Studies of the Chromophores of the Polypeptide Chain Using Excitation below 300 nmChapter 5. Resonance Raman Studies of Natural, Protein-Bound Chromophores I. Introduction II. Heme Proteins III. Vitamin B12 (Cobalamin) IV. Chlorophylls V. Carotenoids VI. The Visual Pigments and Bacteriorhodopsin VII. Flavin Nucleotides VIII. MetalloproteinsChapter 6. Resonance Raman Labels I. Introduction II. Drug-Protein Complexes and Other Enzyme-Inhibitor Systems III. Antibody-Hapten Complexes IV. Permanently Labeled Sites-Arsanilazocarboxypeptidase A V. Enzyme-Substrate Reactions VI. Nucleic Acids and MembranesChapter 7. Nucleic Acids and Nucleic Acid-Protein Complexes I. The Structure of Nucleic Acids II. Polynucleotide Structure from the Normal Raman Spectrum III. Applications to RNA and DNA Structures IV. Viruses and Other Nucleic Acid-Protein Complexes V. Resonance Raman Studies of Nucleic AcidsChapter 8. Lipids, Membranes, and Carbohydrates I. Structures of Lipids and Membranes II. Raman Spectra of Lipids and Membranes III. CarbohydratesSuggestions for Further ReadingReferencesIndex
