Nuclear Particles

List of ContributorsPrefaceContents of Other VolumesChapter 1 Nuclear 30 S RNP Particles I. Isolation and Characterization of Nuclear RNP Particles II. Characteristics of the Protein and RNA Moieties of 30 S Particles III. Structure of Nuclear RNP Complexes IV. Conclusion: Structural Organization ReferencesChapter 2 The Composition and General Topology of RNA and Protein in Monomer 40 S Ribonucleoprotein Particles I. Introduction II. Composition of Monomer 40 S hnRNP Particles III. The Arrangement of RNA and Protein in Monomer 40 S hnRNP Particles IV. Summary and a Possible Mechanism for 40 S hnRNP Function in RNA Splicing ReferencesChapter 3 Two-Dimensional Gel Electrophoresis of Nuclear Particles I. Introduction II. Two-Dimensional Gel Electrophoresis Procedure III. Standardization of Gels IV. Saline-EDTA and Tris Washers of Nuclei V. Nuclear Matrix Proteins VI. Nuclear Membrane-Lamina Complex VII. Nucleolar Matrix VIII. Proteins of hnRNA IX. Proteins of Whole Nuclei and Chromatin X. Association of hnRNP with the Nuclear Matrix XI. Possible Gene Duplication of NHP XII. Silver Staining of Two-Dimensional Gels XIII. Summary ReferencesChapter 4 Immunocytochemistry of Nuclear hnRNP Complexes I. hnRNP Complexes II. Localization of 30 S RNP Core Proteins III. Summary: hnRNP Core Proteins and Nuclear RNA Metabolism ReferencesChapter 5 Enzymatic Activities Associated with hnRNP I. Introductory Remarks II. Enzymes Involved in Posttranscriptional Maturation of hnRNA into mRNA III. Enzymes Involved in Posttranslational Modifications of hnRNP Proteins IV. Concluding Remarks ReferencesChapter 6 Digestion Products of Nuclear Ribonucleoprotein I. Introduction II. Initial Work Utilizing Nucleases III. Studies Involving Specific Nucleotide Sequences IV. Concluding Remarks ReferencesChapter 7 Nucleocytoplasmic Transport of mRNA I. Introduction II. Processing as a Prerequisite for Transport III. Transport through the Nuclear Pore IV. Evidence for Regulation of mRNA Transport V. Evidence for Defective Regulation of mRNA Transport VI. Possible Mechanism of Regulation-mRNA-Associated Proteins References Chapter 8 Nucleocytoplasmic Transport of Ribosomal Subparticles: Interplay with the Nuclear Envelope I. Introduction II. Tetrahymena: Nuclear System and Extrachromosomal rRNA Genes III. Nucleocytoplasmic rRNP Transport IV. The Nuclear Envelope V. rRNP Translocation through Pore Complexes VI. Conclusion References Chapter 9 RNP Particles Involved in Release of in Vitro Synthesized Poly(A)-Containing RNA in Isolated Nuclei I. Introduction II. Results and Discussion ReferencesChapter 10 Nuclear Bodies as Functional Indicators in the Target Cells of Sex Steroid Hormones I. Introduction II. Effects of Estrogens on Estrogen-Induced Responses and Estrogen Receptor Binding in Relation to the Formation of Nuclear Bodies III. Evidence Related to Endocrine Control of Nuclear Body Formation in the Uterus IV. Some Tentative Conclusions and Speculations Concerning the Structure and Function of Nuclear Bodies ReferencesChapter 11 Nuclear Glycoproteins and Glycosaminoglycans I. Evidence for the Presence of Nuclear Glycoproteins and Glycosaminoglycans II. Association of Carbohydrate-Containing Molecules with Nucleoli and Nucleosomes III. Functional Implications of Nuclear Glycoproteins and Glycosaminoglycans ReferencesIndex