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The Cell Nucleus, Volume III: Nuclear Particles, Part A covers topics on "splicing" and "processing" and on the controls of transcriptional and transport events that must be essential to cells that are either growing and/or are phenotypically differentiated. The book discusses the immunolocalization and structural organization of nascent RNP; the in situ localization of RNA structures; and the morphogenesis, cytochemistry, and putative role of perichromatin granules. The text describes transcription in the isolated nuclei; the isolation and structure of the ribonucleoprotein fibrils containing heterogeneous nuclear RNA; and low-molecular-weight nuclear ribonucleoprotein particles. The sequence, function, and role of U snRNAs of nuclear snRNPs; the maturation of low-molecular-weight RNA species; and the properties of the heterogeneous nuclear RNA-protein complexes and nuclear matrix are also encompassed. The book further tackles human antibodies to RNA-containing particles. Cellular biologists, microbiologists, pharmacologists, geneticists, and students taking related courses will find the book useful.
Language
Place of publication
Publishing group
Elsevier Science & Techn.
ISBN-13
978-1-4832-1680-5 (9781483216805)
Schweitzer Classification
Contributors Preface Contents of Other Volumes Chapter 1 Immunolocalization and Structural Organization of Nascent RNP I. Introduction II. Transcriptional Activities III. Interaction of Nascent RNA Transcripts with Proteins IV Immunological Studies on Nascent RNP Transcripts V Ultrastructural Analysis of Nascent RNP Transcripts VI. Functional Activities of Nascent RNP Transcripts ReferencesChapter 2 In Situ Localization of RNA Structures I. Introduction II. Methods in Ultrastructural Detection of RNA III. General Organization of Nuclear RNA Structures IV The Border of Condensed Chromatin V. RNA Structures Present in the Interchromatin Space VI. The Nucleolus VII. Conclusions References Chapter 3 Perichromatin Granules I. Introduction II. Morphogenesis and Fine Structure of the PCG's III. Variations in the Number of PCG's IV. Cytochemistry of PCG's in Situ V. Isolation of the PCG's VI. Putative Role of the PCG's References Chapter 4 Transcription in Isolated Nuclei I. Introduction II. Techniques for Isolating Nuclei III. Transcription in Isolated Nuclei IV. Modification of Transcriptional Activity V. Conclusions References Chapter 5 Isolation and Structure of the Ribonucleoprotein Fibrils Containing Heterogeneous Nuclear RNA I. Introduction II. Isolation of hnRNP III. The Two Classes of Units of hnRNP IV. The Constituents of hnRNP V. Conclusions References Chapter 6 Low-Molecular-Weight Nuclear Ribonucleoprotein Particles I. Introduction II. Isolation and Characterization of Low-Molecular-Weight Nuclear RNP's Containing snRNA Species (snRNP's) III. The Presence of snRNA in hnRNP Complexes IV. Relation of the 10 S snRNP Particles to 30-50 S Structures V. Poly(A)-Containing Low-Molecular-Weight Nuclear RNP Particles VI. Biological Role of snRNP's VII. Conclusions References Chapter 7 U snRNA's of Nuclear snRNP's I. Introduction II. Primary Sequence of snRNA's III. Other Studies on snRNA's IV. Functions of snRNA's V. Structural Homologies of snRNA's References Chapter 8 Maturation of Low-Molecular-Weight RNA Species I. Introduction II. Maturation References Chapter 9 Heterogeneous Nuclear RNA- Protein Complexes and Nuclear Matrix I. Introduction II. Properties of hnRNA-Protein Complexes III. Heterogeneous Nuclear RNA and Small-Molecular-Weight RNA IV. Proteins in nRNP Complexes V. nRNP Complexes and Nuclear Matrix VI. Models VII. Conclusion and Prospects References Chapter 10 Human Antibodies to RNA-Containing Particles I. Introduction II. Ribosomes III. Nucleoli IV. Nuclear RNP Particles References Index