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The Cell Nucleus, Volume I reports the basic concepts of cell nucleus, including nuclear structure, the interaction between the nucleus and cytoplasm, and the chromatin. This volume first describes the nucleus' morphological structures and relates these structures to its functions. It then discusses nuclear organization in plant cells; morphology and biochemistry of the slime mold nucleus; and structure, function, and properties of nuclear envelope. In addition, it addresses the molecular movements between nucleus and cytoplasm against a concentration gradient, presents experiments with animal cell heterokaryons, and explains the genome in specialized cells. It also explores the organization of the chromatin fiber; the human chromosome structure before and after banding; and the ultrastructure and function of heterochromatin and euchromatin.
Language
Place of publication
Publishing group
Elsevier Science & Techn.
ISBN-13
978-0-323-14333-2 (9780323143332)
Schweitzer Classification
List of Contributors Preface Contents of Other Volumes IntroductionPart I Nuclear Structure Chapter 1 Localization of Nuclear Functions as Revealed by Ultrastructural Autoradiography and Cytochemistry I. Localization of Chromatin II. Sites of Replication III. Localization of Ribonucleoproteins IV. Sites of Transcription V. Nuclear Proteins VI. Discussion References Chapter 2 The Nucleolus and Nucleolar DNA I. Historical II. Variations in Nucleolar Structure III. The Role of the Nucleolus IV. The Ultrastructure of the Nucleolus V. Perinucleolar and Intranucleolar Chromatin Structures VI. Compact Nucleoli VII. Nucleoli with Nucleolonemas VIII. Ring-Shaped Nucleoli IX. Nucleolar Fibrillar and Granular Components X. Dense Intranucleolar Particles XI. The Nucleolar Microspherules XII. The Nucleolar Light Areas: Interstices and Vacuoles XIII. Nucleolar Fibrillar Centers and Nucleolini XIV. Natural Nucleolar Segregation XV. Nucleolar Constituents XVI. Synthesis and Cell Content of rDNA XVII. Summary References Chapter 3 Ultrastructural Organization of Plant Cell Nuclei I. Introduction II. Types of Interphase Nuclei III. Organization and Possible Role of Pufflike Chromosome Segments IV. Possible Factors Involved in Nuclear Structure Diversity V. Pattern of Organization of Plant Nucleoli References Chapter 4 The Nucleus of the Plasmodial Slime Molds I. Introduction II. Culture Methods and Gross Morphology III. Microscopic Morphology IV. Chromosome Numbers V. Isolation of Nuclei and Nucleoli VI. Biochemistry References Chapter 5 Structures and Functions of the Nuclear Envelope I. Introduction II. The Nuclear Envelope as a Means of Intracellular ComPartmentalization III. Methodological Progress IV. The Nuclear Envelope as a Part of the Endoplasmic Reticulum V. Associations of Inner Nuclear Membrane with Chromosomes and Chromatin VI. Association of the Inner Nuclear Membrane with Specific Proteinaceous Layers VII. The Pore Complex VIII. Relationship of the Nuclear Envelope to Annulate Lamellae (AL) and Intranuclear Cisternae IX. Structural Differentiation of the Nuclear Envelope X. Associations and Interactions of the Nuclear Envelope with Microtubules and Microfilaments XI. Relationship of the Nuclear Envelope to Other Cell Organelles XII. Breakdowns and Reconstitutions of the Nuclear Envelope; Role of the Nuclear Envelope in Nuclear Divisions and Fusions Note Added in Proof References Chapter 6 Chemical and Biochemical Properties of the Nuclear Envelope I. Introduction II. Isolation and Physical Properties III. Chemical Properties IV. Biochemical Properties V. Conclusions Note Added in Proof ReferencesPart II Nuclear-Cytoplasmic Interactions Chapter 7 Movement of Molecules between Nucleus and Cytoplasm I. Introduction II. Observations from Which It Can Be Inferred That Molecules Move Nonrandomly between Cytoplasm and Nucleus III. Unidirectional Movement of RNA from Nucleus to Cytoplasm IV. Unidirectional Movement of Proteins from Cytoplasm to Nucleus V. Movement of DNA from Nucleus to Cytoplasm VI. Some Proteins Shuttle Nonrandomly between Nucleus and Cytoplasm VII. RNA's That Shuttle Nonrandomly between Nucleus and Cytoplasm VIII. Movement of Ions between Nucleus and Cytoplasm IX.