Guide to Protein Purification
2nd Edition
Published on 3. November 2009
900 pages
978-0-08-092317-8 (ISBN)
Description
Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today.
- Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology
- Assembles chapters on both common and less common relevant techniques
- Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process
More details
Other editions
Additional editions
Richard R. Burgess | Murray P. Deutscher
Guide to Protein Purification: Volume 463
Book
12/2009
2nd Edition
Academic Press
€179.51
Shipment within 15-20 days
Content
1. Why Purify Enzymes?
Section I: Developing Purification Procedures2. Strategies and Considerations for Protein Purifications3. Use of Bioinformatics in Planning a Protein Purification4. Preparing A Purification Summary Table
Section II: General Methods for Handling Proteins and Enzymes5. Setting Up A Laboratory6. Buffers: Principle and Practice7. Measurement of Enzyme Activity8. Quantitation of Protein9. Concentration of Proteins and Removal of Solutes10. Maintaining Protein Stability
Section III: Recombinant Protein Expression and Purification11. Selecting an Appropriate Method for Expressing a Recombinant Protein12. Bacterial Systems for Production of Heterologous Proteins13. Expression in the Yeast Pichia Pastoris14. Baculovirus-Insect Cell Expression Systems15. Recombinant Protein Production By Transient Gene Transfer Into Mammalian Cells16. Tagging For Protein Expression17. Refolding of Solubilized Inclusion Body Proteins
Section IV: Preparation of Extracts and Subcellular Fractionation 18. Advances In Preparation of Biological Extracts for Protein Purification19. Isolation of Subcellular Organelles and Structures
Section V: Purification Procedures: Bulk Methods20. Protein Precipitation Techniques21. Affi-Gel Blue for Nucleic Acid Removal and Early Enrichment of Nucleotide Binding Protein
Section VI: Purification Procedures: Chromatographic Methods22. Ion Exchange Chromatography23. Gel Filtration24. Protein Chromatography on Hydroxyapatite Columns25. Theory and Use of Hydrophobic Interaction Chromatography in Protein Purification Applications
Section VII: Purification Procedures: Affininty Methods26. Affinity Chromatography: General Methods27. Immobilized-Metal Affinity Chromatography (IMAC)28. Identification, Production, and Use of Polyol-Responsive Monoclonal Antibodies for Immunoaffinity Chromatography
Section VIII: Purification Procedures: Electrophoretic Methods29. One-Dimensional Gel Electrophoresis30. Protein Analysis Using High-Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis and Isoelectric Focusing31. Protein Gel Staining Methods: An Introduction And Overview32. Elution of Proteins From Gels33. Immunodetection By Protein Blotting Performing and Optimizing Western Blots With An Emphasis on Chemiluminescent Detection
Section IX: Purification Procedures: Membrane Proteins And Glycoproteins34. Detergents: An Overview35. Purification of Membrane Proteins36. Purification of Recombinant G-Protein-Coupled Receptors37. Cell-Free Translation Of Integral Membrane Proteins Into Unilamelar Liposomes
Section X: Characterization of Purified Proteins38. Determination of Protein Purity39. Determination of Size, Molecular Weight, and Presence of Subunits40. Identification and Quantification of Protein Posttranslational Modifications41. Parallel Methods For Expression And Purification42. Techniques To Isolate O2-Sensitive Proteins: [4Fe-4S]-FNR As An Example43. Rethinking Your Purification Procedure44. Important But Little Known (Or Forgotten) Artifacts in Protein Biochemistry
