Volume I
2nd Edition
Published on 5. October 2000
261 pages
978-0-19-156564-9 (ISBN)
Description
The two Essential Molecular Biology books in the Practical Approach Series are designed for the absolute beginner at gene cloning whether they be at the start of their career or an experienced researcher in another field. As with the first editions, the objective of both volumes is to combine solid practical information with sufficient background material to ensure that the novice can understand how a technique works, what it achieves, and how to make modifications to suit personal requirements. Volume 1 concentrates on the procedures for DNA and RNA manipulation: purification, electrophoresis, and the construction and cloning of recombinant molecules. It also includes a general introduction to molecular biology in the laboratory and a survey of cloning vectors for Escherichia Coli.
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Content
- Getting Started in Molecular Biology
- Microbiological Techniques for Molecular Biology: bacteria and phages
- Purification of DNA
- Purification of RNA
- Nucleic Acid Electrophoresis in Agarose Gels
- Recovery of DNA from Electrophoresis Gels
- Construction of Recombinant DNA Molecules
- Generation and Identification of Recombinant Clones
- Survey of Cloning Vectors for Escherichia coli
- A1: Important Escherichia coli Strains
- A2: Recipes and General Procedures
- A3: Restriction Endonucleases
