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The Cell: Biochemistry, Physiology, Morphology, Volume I focuses on the advancements of processes, techniques, and approaches used in studies on the structure and functions of cells. The selection first offers information on the optical methods in cytology, fixation and staining, and autoradiography. Discussions focus on microscopical analysis, techniques related to autoradiography, staining for electron microscopy and light microscopy, fixation, electron microscope in cytology, and X-ray microscopy of cells. The text then takes a look at the quantitative microscopical techniques for single cells and quantitative microchemical techniques of histo- and cytochemistry. The manuscript ponders on micrurgical studies on living cells, isolation of subcellular components, and "tissue culture," cellular autonomy, and cellular interrelations. Topics include standardization procedures, factors influencing the choice of isolation procedure, future applications and extensions of micrurgy, and applications of micrurgy to the study of living cells. The effects of radiations on cells, acquisition of biological specificity, and nucleocytoplasmic interactions in eggs and embryos are also discussed. The selection is a dependable reference for researchers interested in pursuing further studies on the biochemistry, physiology, and morphology of cells.
Language
Place of publication
Publishing group
Elsevier Science & Techn.
ISBN-13
978-1-4832-1654-6 (9781483216546)
Schweitzer Classification
¿List of ContributorsPrefaceContents, Volumes II AND IIIContents, Volumes IV AND VIntroductionPart I: Methods 1. Optical Methods in Cytology I. Introduction II. Extensions of Optical Microscopy III. The Electron Microscope in Cytology IV. The X-Ray Microscopy of Cells V. Conclusion References 2. Fixation and Staining I. Definition of Fixation and Staining II. Fixation III. Staining for Light Microscopy IV. Staining for Electron Microscopy References 3. Autoradiography I. Introduction II. Outline of the Various Autoradiography Techniques III. Techniques Related to Autoradiography IV. Microscopical Analysis V. Conclusion References 4. Quantitative Microscopical Techniques for Single Cells I. Introduction II. Methods III. Individual Techniques IV. Conclusions References 5. Quantitative Microchemical Techniques of Histo- and Cytochemistry I. Introduction II. Preparation of Samples III. Measurement of Amount of Sample IV. Titrimetric Techniques V. Spectrophotometric Techniques VI. Flame Photometric Techniques VII. Fluorometric Techniques VIII. Gasometric Techniques IX. Dilatometric Techniques X. Microbiological Techniques XI. Conclusions References 6. Micrurgical Studies on Living Cells I. Historical II. Instrumentation and Procedures III. Application of Micrurgy to the Study of Living Cells IV. Subcellular Transplantation V. Future Applications and Extensions of Micrurgy References 7. The Isolation of Subcellular Components I. Introduction II. Factors Influencing the Choice of Isolation Procedure III. The Techniques of Cell Fractionation IV. Some Representative Isolation Procedures V. Standardization Procedures VI. Some General Conclusions References 8. The Cell as Organism. "Tissue Culture," Cellular Autonomy, and Cellular Interrelations I. Introduction II. History III. The Subject Matter IV. Methods V. Conclusions ReferencesPart II: Problems of Cell Biology 9. Fertilization I. General Aspects of Fertilization II. Fertilization in Selected Cases III. Conclusions References 10. Sex Determination I. Introduction II. Cytogenetic Theory of Sex Determination III. Karyologic Basis of the Mechanisms of Sex Determination IV. Experimental Verifications of the Cytogenetic Theory of Sex Determination V. Genetic Bipotentiality of Sex VI. Intersexuality and Genetic Interpretation of Sex: Genic Balance VII. Epigamous Factors, Intersexuality and Sex Determination VIII. Conclusions References 11. Differentiation of Vertebrate Cells I. The Concept of Differentiation II. Criteria of Differentiation III. Properties of Cytodifferentiation IV. Theoretical Considerations References 12. Patterns of Cell Growth and Differentiation in Plants I. Introduction II. Shoot Development from a Single Apical Cell III. Apical Development from a Group of Initial Cells References 13. Nucleocytoplasmic Interactions in Eggs and Embryos I. Introduction II. Organization of the Egg III. Nucleocytoplasmic Interactions in Cleavage IV. Nucleocytoplasmic Interactions in Differentiation V. General Summary References 14. The Acquisition of Biological Specificity I. Biological Specificity II. Transplantation Immunity III. The Ontogeny of Transplantation Immunity IV.