Methods of Enzymatic analysis V4
2nd Edition
Published on 2. December 2012
800 pages
978-0-323-16132-9 (ISBN)
Description
Methods of Enzymatic Analysis, Volume 4 reviews developments in the use of enzymes as tools in analytical biochemistry, including advances in assay techniques. It discusses the principles and methods for the elucidation of structures of enzymes, such as peptides, proteins, amino acids, fatty acid metabolites, lipids, steroids, nucleic acids, purines, pyrimidines, nucleosides, and coenzymes. It also considers the isolation and characterization of active centers in enzymes. This volume is divided into four parts, each discussing a group of enzymes and their determination. Part I focuses on proteins, peptides, and amino acids including amines and amides. Part II is concerned with fatty acid metabolites, lipids, and steroids ranging from polyunsaturated fatty acids and lecithin to choline, acetylcholine, triglycerides, glycerol, acetoacetate, triacetate, fumarylacetoacetate, 20-ketosteroids, prostaglandins, bile acids, and cholesterol. Part III discusses nucleic acids, purines, pyrimidines, nucleosides, coenzymes, and related compounds, whereas Part IV looks at other substrates and effectors such as inorganic phosphate. The book concludes with a chapter on metabolites and their concentrations in animal tissues. Biochemists as well as students and researchers working in the field of analytical biochemistry will find this book highly informative.
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Hans Ulrich Bergmeyer
Methods of Enzymatic Analysis: v. 4
Book
02/1975
2nd Edition
Academic Press
€53.97
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Content
PrefaceFrom the Preface to the 1st EditionVolume IContentsContributorsAbbreviationsSection A: General Introduction I. The Importance of Enzymatic Analysis 1. in Biochemistry 2. in Medicine 3. in Foodstuff Chemistry 4. in Botany and Agricultural Chemistry 5. in Microbiology II. Principles of Enzymatic Analysis 1. Introduction 2. Reaction Kinetics 3. Determination of Concentration of Metabolites (End-point Methods) 4. Determination of Enzyme Activities 5. Determination of Concentrations by Kinetic Methods 6. Kinetics of "Enzymatic Cycling" 7. Visualization of NAD(P)-dependent Reactions 8. Determination of Michaelis Constants and Inhibitor Constants III. Experimental Techniques 1. Handling of Biochemical Reagents and Samples 2. Methods of Measurement and Instruments Used 3. Evaluation of Experimental Results IV. Statistical Analysis, Control and Assessment of Experimental Results 1. Introduction 2. Statistical Concepts and Methods 3. Quality Control 4. Assessment of Results V. Cell and Tissue Disintegration 1. General 2. Methods for Animal Tissue and Micro-organisms 3. Methods for Plant TissuesSection B: Biochemical Reagents I. Reagents for Enzymatic Analysis 1. Nomenclature and Standardization 2. Quality Requirements 3. Suppliers of Biochemical Reagents II. Enzymes as Biochemical Reagents III. Coenzymes, Metabolites and other Biochemical Reagents IV. Complete Reagent Kits 1. For Determination of Metabolites 2. For Determination of Enzyme Activities 3. Main Manufacturers and SuppliersIndexVolume IIContentsContributorsAbbreviationsSection C: Methods for Determination of Enzyme Activity I. Oxidoreductases 1. Sorbitol Dehydrogenase 2. Lactate Dehydrogenases 3. Malate Dehydrogenase 4. Isocitrate Dehydrogenase 5. 6-Phosphogluconate Dehydrogenase 6. Glucose-6-phosphate Dehydrogenase 7. Xanthine Oxidase 8. Glutamate Dehydrogenase 9. Diamine Oxidase 10. Dihydrofolate Reductase (Tetrahydrofolate Dehydrogenase) 11. Catalase 12. Peroxidases II. Transferases 1. Ornithine Carbamoyltransferase 2. Transamidinase 3. Transketolase 4. Transaldolase 5. ¿-Glutamyltranspeptidase 6. UDP-Glucuronyltransferase 7. Aminotransferases and Related Enzymes 8. Phosphotransferases III. Hydrolases 1. Esterases 2. Glycoside Hydrolases 3. Peptidases and Proteinases 4. Other C-N splitting Hydrolases IV. Lyases, Isomerases, Ligases 1. Fructose-1,6-diphosphate Aldolase 2. 1-Phosphofructoaldolase 3. Glucosephosphate Isomerase 4. Tetrahydrofolate FormylaseIndexVolume IIIContentsContributorsAbbreviationsSection D. Methods for Determination of Metabolites I. Carbohydrate Metabolites 1. Poly-, Oligo- and Disaccharides 2. Monosaccharides and Derivatives 3. Three Carbon compounds 4. Two and One Carbon Compounds II. Citric Acid Cycle Metabolites 1. Citrate 2. Isocitrate 3. 2-Oxoglutarate 4. L-(-)-Malate 5. L-Malate and Fumarate 6. Oxaloacetate 7. Succinate 8. MaleateIndexVolume IVContentsContributorsAbbreviations III. Proteins, Peptides and Amino Acids 1. Proteins and Peptides 2. Amino Acids, Amines and Amides IV. Fatty Acid Metabolites, Lipids and Steroids 1. Polyunsaturated Fatty Acids 2. Lecithin 3. Acetylcholine and Choline 4. Triglycerides and Glycerol Determination after Alkaline Hydrolysis 5.
