Applications of Chimeric Genes and Hybrid Proteins, Part A: Gene Expression and Protein Purification
Gene Expression and Protein Purification
Academic Press
Published on 11. October 2000
617 pages
978-0-08-049681-8 (ISBN)
Description
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
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Jeremy Thorner | Scott D. Emr | John N. Abelson
Applications of Chimeric Genes and Hybrid Proteins, Part A: Gene Expression and Protein Purification: Volume 326
Book
10/2000
Academic Press
€167.41
Withdrawn from sale
Persons
Content
- Front Cover
- Aapplications of Chimeric Genes and Hybrid Proteins
- Copyright Page
- Table of Contents
- Contributors to Volume 326
- Preface
- Volumes in Series
- Section I: Historical Overview
- Chapter 1. The All Purpose Gene Fusion
- Section II: Gene Fusions as Reporters of Gene Expression in Prokaryotic Cells
- Chapter 2. A Practical Guide to the Construction and Use of lac Fusions in Escherichia coli
- Chapter 3. Tagging Exported Proteins Using Escherichia coli Alkaline Phosphatase Gene Fusions
- Chapter 4. Applications of Gene Fusions to Green Fluorescent Protein and Flow Cytometry to the Study of Bacterial Gene Expression in Host Cells
- Chapter 5. IVET and RIVET: Use of Gene Fusions to Identify Bacterial Virulence Factors Specifically Induced in Host Tissues
- Chapter 6. Identification of Exported Bacterial Proteins via Gene Fusions to Yersinia pseudotuberculosis Invasin
- Section III: Gene Fusions as Reporters of Gene Expression in Eukaryotic Cells
- Chapter 7. Use of Imidazoleglycerolphosphate Dehydratase (His3) as a Biological Reporter in Yeast
- Chapter 8. Use of Fusions to Human Thymidine Kinase as Reporters of Gene Expression and Protein Stability in Saccharomyces cerevisiae
- Chapter 9. Use of Fusions to Thymidine Kinase
- Chapter 10. In Vivo Analysis of lacZ Fusion Genes in Transgenic Drosophila melanogaster
- Chapter 11. Utility of the Secreted Placental Alkaline Phosphatase Reporter Enzyme
- Chapter 12. Fusions to Imidazopyrazinone-Type Luciferases and Aequorin as Reporters
- Chapter 13. Novel Methods for Chemiluminescent Detection of Reporter Enzymes
- Chapter 14. Fusions to Chloramphenicol Acetyltransferase as a Reporter
- Chapter 15. Fusions to ß-Lactamase as a Reporter for Gene Expression in Live Mammalian Cells
- Section IV: Functional Tags for Biochemical Purification
- Chapter 16. Purification of Proteins Using Polyhistidine Affinity Tags
- Chapter 17. Generating Fusions to Glutathione S-Transferase for Protein Studies
- Chapter 18. Use of the Strep-Tag and Streptavidin for Detection and Purification of Recombinant Proteins
- Chapter 19. Streptavidin-Containing Chimeric Proteins. Design and Production
- Chapter 20. Fusions to Maltose-Binding Protein: Control of Folding and Solubility in Protein Purification
- Chapter 21. Thioredoxin as a Fusion Partner for Production of Soluble Recombinant Proteins in Escherichia coli
- Chapter 22. Affinity Purification of Recombinant Proteins Fused to Calmodulin or to Calmodulin-Binding Peptides
- Chapter 23. The S · Tag Fusion System for Protein Purification
- Chapter 24. Fusions to Self-Splicing Inteins for Protein Purification
- Chapter 25. Fusion Proteins Containing Cellulose-Binding Domains
- Chapter 26. Biotinylation of Proteins in Vivo and in Vitro Using Small Peptide Tags
- Chapter 27. Biotinylation of Proteins in Vivo: A Useful Posttranslational Modification for Protein Analysis
- Section V: Hybrid Proteins for Detection and Production of Antigens and Antibodies
- Chapter 28. Methods for Generating Multivalent and Bispecific Antibody Fragments
- Chapter 29. Design and Use of Phage Display Libraries for the Selection of Antibodies and Enzymes
- Chapter 30. Use of an Lpp-OmpA Fusion Vehicle for Bacterial Surface Display
- Chapter 31. Identification of Bacterial Class I Accessible Proteins by Disseminated Insertion of Class I Epitopes
- Chapter 32. Bordetella pertussis Adenylate Cyclase Toxin: A Vehicle to Deliver CD8-Positive T-Cell Epitopes into Antigen-Presenting Cells
- Chapter 33. Use of Anthrax Toxin Fusions to Stimulate Immune Responses
- Chapter 34. Use of Fusions to Viral Coat Proteins as Antigenic Carriers for Vaccine Development
- Author Index
- Subject Index
