
Advances in Bio-Fuel Production
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Content
- Intro
- Contents
- Preface
- Acknowledgements
- Chapter 1
- Introduction to Biofuels Production
- Abstract
- Introduction
- Acknowledgments
- References
- Websites
- Chapter 2
- Importance of Cellulosic Bioethanol and Pre-Treatment Methods for Separating Lignin from Lignocellulosic Biomass
- Abstract
- 2.1. Introduction
- 2.2. Methods of Pretreatment
- 2.2.1. Physical Pretreatment
- 2.2.1.1. Mechanical Extrusion
- 2.2.1.1.1. Procedure 1
- 2.2.1.1.2. Procedure 2
- 2.2.1.1.3. Procedure 3
- 2.2.1.1.4. Procedure 4
- 2.2.1.1.5. Procedure 5
- 2.2.2. Chemical Pretreatment
- 2.2.2.1. Procedure 1
- 2.2.2.2. Procedure 2
- 2.2.2.3. Procedure 3
- 2.2.2.4. Procedure 4
- 2.2.2.5. Procedure 5
- 2.2.2.6. Procedure 6
- 2.2.2.7. Procedure 7
- 2.2.2.8. Procedure 8
- 2.2.2.9. Procedure 9
- 2.2.2.10. Procedure 10
- 2.2.3. Thermochemical Pretreatment
- 2.2.3.1. Procedure 1
- 2.2.3.2. Procedure 2
- 2.2.3.3. Procedure 3
- 2.2.3.4. Procedure 4
- 2.2.3.5. Procedure 5
- 2.2.4. Ionic Liquid Fractionation
- 2.2.4.1. Procedure 1
- 2.2.4.2. Procedure 2
- 2.2.4.3. Procedure 3
- 2.2.4.4. Procedure 4
- 2.2.4.5. Procedure 5
- 2.2.5. Biological Pretreatment
- 2.2.5.1. Procedure 1
- 2.2.5.2. Procedure 2
- 2.2.5.3. Procedure 3
- 2.2.5.4. Procedure 4
- 2.2.6. Co-Solvent Fractionation
- Conclusion
- Acknowledgments
- References
- Chapter 3
- Hexose Fermentation for Ethanol Production
- Abstract
- 3.1. Introduction
- 3.2. Glucose Utilization in the Metabolic Pathways
- 3.3. Micro-Organisms Involved in Hexose Fermentation
- 3.4. Ethanol Fermentation
- 3.5. Lactic Acid Fermentation
- 3.6. Heterolactate Fermentation
- 3.7. Butanol/Butyric Acid Fermentation
- 3.8. Formic Acid Fermentation
- 3.9. Butanediol Fermentation
- 3.10. Mixed Acids Fermentation
- 3.11. Propionic Acid Fermentation
- Conclusion
- Acknowledgments
- References
- Chapter 4
- Biofuel Production from Pentose Sugars
- Abstract
- 1. Introduction
- 2. Pentose Sugars
- 3. Sources of Pentose Sugars
- 4. Pre-Treatment of Hemicelluloses
- 4.1. Dilute Acid
- 4.2. Alkali
- 4.3. Sulfur Dioxide (SO2)
- 4.4. Hydrogen Peroxide
- 4.5. Steam Explosion
- 4.6. Ammonia Fiber Explosion (AFEX)
- 4.7. Wet Oxidation
- 4.8. Lime
- 4.9. Liquid Hot Water
- 4.10. CO2/ Freeze Explosion
- 5. Bioconversion of Pentose Sugars into Ethanol
- 6. Biofuel from D-Xylose
- 7. Biofuel from Arabinose
- 8. Pentose Fermenting Microorganisms
- 9. Filamentous Fungi
- 10. Bacteria
- 11. Yeasts
- 12. Strain Improvement
- 13. Mutation
- 14. Protoplast Fusion
- 15. Genetic Engineering
- Conclusion
- References
- Chapter 5
- Genetic and Metabolic Engineering of Microbes for Efficient Lignocellulosic Ethanol
- Abstract
- 5.1. Introduction
- 5.2. LCB as Substrate for Biofuel Production- Benefits, and Challenges
- 5.3. Potential Ethenologenic Microoganisms
- 5.4. Strategies to Improve Xylose Utilization for Ethanol Production
- 5.4.1. Non-Targeted Engineering
- 5.4.1.1. Evolutionary Engineering
- 5.4.1.2. Enhancement of Xylose Uptake
- 5.4.1.3. Enhancement of Stress Tolerance
- 5.4.1.4. Strain Hybridization
- 5.4.1.4.1. Protoplast Fusion
- 5.4.1.4.2. Genome Shuffling
- 5.4.2. Targeted Engineering
- 5.4.2.1. Engineering XR/XDH Pathway
- 5.4.2.2. Engineering XI Pathway
- 5.4.2.3. Engineering Non-Oxidative Pathway
- Conclusion
- References
- Chapter 6
- Microalgae Based Biofuels: Production, Improvement, Processing and Extraction
- Abstract
- 6.1. Introduction
- 6.2. Potential Role of Microalgal Biodiesel
- 6.3. Technologies for Microalgal Cultivation
- 6.3.1. Photoautotrophic Cultivation
- 6.3.1.1. Open Pond Cultivation
- 6.3.1.1.1. Unstirred Open Ponds
- 6.3.1.1.2. Circular Ponds
- 6.3.1.1.3. Raceway Ponds
- 6.3.2. Closed Photobioreactor Systems
- 6.3.2.1. Tubular Photobioreactor
- 6.3.2.2. Flat-Plate Photobioreactor
- 6.3.2.3. Vertical Column Photobioreactors
- 6.3.3. Hybrid Production Systems
- 6.3.4. Heterotrophic Production
- 6.3.5. Mixotrophic Production
- 6.4. Improvement Strategies for Microalgae Fuel Production
- 6.4.1. Strain Selection
- 6.4.2. Metabolic Engineering Approach
- 6.4.3. In silico Metabolic Engineering
- 6.4.4. Genetic Engineering
- 6.4.4.1. Transformation Techniques
- 6.4.4.2. Transgenic Microalgal Strains, Stability, and Antibiotic R esistant
- 6.4.4.3. CRISPR Technology for Genome Editing in Microalgae
- 6.5. Processing of Biofuels
- 6.5.1. Thermochemical Processing
- 6.5.1.1. Thermochemical Liquefaction
- 6.5.1.2. Gasification
- 6.5.1.3. Pyrolysis
- 6.5.2. Biochemical Processing
- 6.5.2.1. Transesterification
- 6.5.2.2. Fermentation
- 6.5.2.3. Anaerobic Digestion
- 6.5.2.4. Hydroprocessing
- 6.6. Extraction of Biofuels
- 6.6.1. Cell Disruption Methods for Lipid Extraction
- 6.6.1.1. Mechanical Methods
- 6.6.1.2. Bead Beating
- 6.6.1.3. Microwave
- 6.6.1.4. Ultrasonication
- 6.6.1.5. High-Pressure Homogenization
- 6.6.1.6. Electroporation
- 6.6.2. Chemical Method
- 6.6.2.1. Chemical Treatments
- 6.6.2.2. Osmotic Shocks
- 6.6.3. Biological Method
- 6.6.4. Total Lipid Extraction Methods
- 6.6.4.1. Folch Method
- 6.6.4.2. Bligh and Dyer Method
- 6.6.4.3. Extraction of All Classes of Lipids
- 6.7. Economics of Biodiesel Production
- Conclusion
- References
- Chapter 7
- Prospects and Problems in the Bioconversion of Hemicellulose of Agro-Residues into Ethanol
- Abstract
- 7.1. Introduction
- 7.2. Hemicelluloses
- 7.3. Hemicellulose-Hydroyzing Microbial Enzymes
- 7.3.1. Xylanases
- 7.3.2. a-L-Arabinofuranosidase (E.C. 3.2.1.55) (AFase)
- 7.3.3. a-D-Glucuronidase (E.C. 3.2.1.139)
- 7.3.4. ß-Mannanases
- 7.3.5. Acetylxylan esterases
- 7.4. Effect of Enzyme Dose and Pretreatment Methods
- 7.5. Impact of Lignocellulose Pretreatment Method
- 7.6. Enzyme Production
- 7.7. Methods for Improving Enzyme Hydrolysis
- 7.8. 'Omic' Approach for Studying Hemicellulases
- 7.9. Synergistic Action of Hemicellulases
- 7.10. Naturally Occurring Pentose Fermenting Microorganisms
- 7.11. Xylose Metabolizing Pathways
- 7.12. Strain Improvement for Pentose Fermentation
- 7.13. Metabolic Engineering of S. Cerevisiae for Efficient Pentose Fermentation
- 7.14. Consolidated Biomass Processing (CBP)
- Future Perspectives and Conclusion
- Acknowledgments
- References
- Chapter 8
- Recent Advancements in the Engineering of ß-Glucosidase for Biomass Degradation
- Abstract
- 8.1. Introduction
- 8.2. Distribution and Function
- 8.3. Classification
- 8.4. Applications of ß-Glucosidase
- 8.5. Structural Diversity in ß-Glucosidase
- 8.6. ß-Glucosidase Substrate Recognition, Binding, Hydrolysis and Product Release
- 8.6.1. Substrate Recognition
- 8.6.2. Molecular Basis of the Glycone Specificity
- 8.6.3. Molecular Basis of the Aglycone Specificity
- 8.7. Activation of ß-Glucosidase by Glucose
- 8.7.1. Transglycosylation of Glucose
- 8.7.2. Glucose Binding to an Allosteric Regulatory Binding Site
- 8.7.3. Glucose Relieves Enzyme from Substrate Inhibition
- 8.8. Inhibition by Substrate and Product
- 8.8.1. Inhibition by Product
- 8.8.2. Types of Product Inhibition in BGs
- 8.9. Glucose Tolerant ß-Glucosidase
- 8.10. GH1 BGs Are More Glucose Tolerant Than GH3 BGs
- 8.11. Role of Enzyme Engineering
- 8.11.1. Rational Design
- 8.11.2. Directed Evolution
- 8.11.3. Transgenic Approaches
- 8.11.4. Examples of Engineering Better ß-Glucosidase
- 8.12. Future Work
- Acknowledgments
- References
- Chapter 9
- Fuels from Photosynthesis for Combustion Engines
- Abstract
- 9.1. Introduction
- 9.2. Calculation of the Energy Content of Photosynthesis Products
- 9.2.1. The Chemical Sign Language
- 9.2.2. Calculation of Energy Content
- 9.3. Fuel Requirements by the Internal Combustion Engine
- 9.3.1. Combustion Kinetics
- 9.3.2. Modern Combustion Engines
- 9.3.3. Spark Ignition and/or Self-Ignition in Internal Combustion Engines
- 9.3.4. Economic Evaluation of Internal Combustion Engines with the Use of Different Fuels
- 9.4. Conversion of Photosynthesis Products into Fuels
- 9.4.1. Fuel from Photosynthesis Products
- 9.4.2. Direct Oil Production of 4th Generation Oil
- 9.4.3. Biogas Production by Wet and Dry Fermentation
- 9.4.4. Gases from Thermal Gasification of 4th Generation Energy Products
- 9.4.5. Alcoholic Fermentation of 4th Generation Energy Products
- 9.4.6. Fuels from 4th Generation Solids
- 9.5. Economic Considerations on Fuels from Natural Products of Photosynthesis
- 9.5.1. Fuel Energy from Fats and Proteins
- 9.5.2. Fuel Energy from Carbohydrates by Means of Thermal Gasification
- 9.5.3. Fuel Energy from Land and Water Plants
- 9.6. Future Prospects for These Fuels
- References
- Websites:
- About the Editors
- Index
- Blank Page
- Blank Page
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