DNA Repair, Part A

Chapter 1 Assays for determining lesion bypass efficiency and mutagenicity of site-specific DNA lesions in vivo Chapter 2 Oxidative DNA glycolases: Recipes from Cloning to Characterization Chapter 3 Purification and Characterization of NEIL1 and NEIL2, Members of a distinct Family of Mammalian DNA Glycosylases for repair of oxidized bases Chapter 4 Analysis of Base Excision DNA Repair of the Oxidative Lesion 2-Deoxyribonolactone and the Formation of DNA-Protein Cross-links Chapter 5 Isolation and analyses of MutY and MutY Homologs (MYH) Chapter 6 Use of yeast for detection of endogenous abasic legions, their source and their repair Chapter 7 Activities and Mechanism of Pol Beta Chapter 8 Direct removal of aklylation damage from DNA by AlkB and related DNA dioxygenases Chapter 9 Purification and Characterization of DNA Photolyases Chapter 10 Genetic and in vitro assays of DNA deamination Chapter 11 The XPC complex and UV-DDB: Functional assays for the damage recognition factors involved in global genome repair Chapter 12 Purification and Characterization of E. coli and Human Nucleotide Excision Repair Chapter 13 Assays for transcription elongation by RNA polymerase II using oligo (dC)-tailed template with single DNA damage Chapter 14 In vivo assays for transcription-coupled repair Chapter 15 TFIIh enzymatic activities in transcription and nucleotide excision repair Chapter 16 An assay for studying ubiquitylation of RNA polymerase II and other proteins in crude yeast extracts Chapter 17 Analysis of the excision step in human DNA mismatch repair Chapter 18 Characterisation of the "mismatch repairosome and its role in the processing of modified nucleosides in vitro Chapter 19 Analysis of DNA Mismatch Repair in Cellular Response to DNA Damage Chapter 20 Characterization of E. coli Translesion Synthesis Polymerases and Their Accessory Factors Chapter 21 Measuring the Fidelity of Translesion DNA Synthesis Chapter 22 DNA Polymerases for Translesion DNA Synthesis: Enzyme Purification and Mouse Models for Studying Their Function Chapter 23 Purification and Characterization of Escherichia coli DNA Polymerase V Chapter 24 Yeast and Human Translesion DNA Synthesis Polymerases: Expression, Purification, and Biochemical Characterization Chapter 25 Localisation of Y-family polymerases and the DNA polymerase switch in mammalian cells Chapter 26 Repair of DNA Double Strand Breaks - in vivo Biochemistry Chapter 27 Assays for nonhomologous end-joining in extracts Chapter 28 Purification and assays of Saccharomyces cerevisiae homologous recombination proteins Chapter 29 Analysis of DNA recombination and repair proteins in living cells by photobleaching microscopy Chapter 30 Synthetic Junctions as Tools to Identify and Characterize Holliday Junction Resolvases Chapter 31 In Vitro Nonhomologous End Joining Assay Chapter 32 The RAG and HMGB1 proteins: purification and biochemical analysis of recombination signal complexes Chapter 33 Purification and Biochemical Characterization of ATM and MRN