The Composition, Structure and Reactivity of Proteins
A Laboratory Manual of Analytical Methods of Protein Chemistry (Including Polypeptides)
Published on 5. June 2014
530 pages
978-1-4831-8453-1 (ISBN)
Description
The Composition, Structure and Reactivity of Proteins
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Content
- Front Cover
- The Composition, Structure and Reactivity of Proteins
- Copyright Page
- Dedication
- Table of Contents
- PREFACE
- CONTENTS LIST: VOLUME 1
- CONTENTS LIST: VOLUME 3
- Chapter 1. AMINO ACID ANALYSIS OF PROTEIN HYDROLYSATES
- A. PREPARATION OF THE SAMPLE FOR ANALYSIS
- B. HYDROLYSIS OF PROTEINS
- C. PAPER CHROMATOGRAPHY
- D. COLUMN CHROMATOGRAPHY ON ION EXCHANGE RESINS
- E. COLORIMETRIC METHODS
- F. MICROBIOLOGICAL METHODS
- G. MISCELLANEOUS METHODS
- REFERENCES
- CHAPTER 2. THE ESTIMATION OF SIDE CHAIN GROUPS IN THE INTACT PROTEIN
- 1A. Total Primary Amino Groups
- 1B. The Reactive Primary Amino Groups
- 2. Amide Groups
- 3. Sulphydryl Groups
- 3A. Available Sulphydryl Groups
- 3B. Total Sulphydryl Content
- 4. The Disulphide Bond
- 5. The Phenolic Groups
- 6. The Imidazole Group
- 7. The Indole Groups
- 8. The Guanidyl Groups
- 9. The Carbonyl Content of Modified Proteins
- REFERENCES
- CHAPTER 3. DEUTERIUM AND 18O EXCHANGE
- INTRODUCTION
- DEFINITIONS
- OUTLINE OF THE METHOD
- THE DENSITY GRADIENT
- EQUIPMENT FOR EXCHANGE EXPERIMENTS
- PROCEDURE
- CALCULATION
- LIMITATIONS AND ERRORS OF THE METHOD
- EXAMPLES
- INTERPRETATIONS
- THE EQUILIBRATION METHOD
- EXCHANGE OF 18O
- REFERENCES
- CHAPTER 4. DYE-BINDING METHODS
- I. INTRODUCTION
- II. PRINCIPLES
- III. EXPERIMENTAL PROCEDURES
- IV. FURTHER METHODS OF STUDYING DYE BINDING
- V. CONCLUSION
- REFERENCES
- CHAPTER 5. ELECTROPHORESIS OF PROTEINS IN LIQUID MEDIA
- I. INTRODUCTION
- II. DESCRIPTION OF APPARATUS
- III. ELECTROPHORETIC MIGRATION
- IV. OPTICAL METHODS
- V. MOVING BOUNDARIES
- VI. MOBILITY AND ISOELECTRIC POINT
- VII. SEPARATIONS OF MIXTURES INTO INDIVIDUAL COMPONENTS
- VIII. ELECTROPHORETIC HOMOGENEITY
- REFERENCES
- CHAPTER 6. PAPER ELECTROPHORESIS
- HISTORICAL
- PRINCIPLE
- PRACTICAL DETAILS
- REFERENCES
- CHAPTER 7. THE TECHNIQUE OF IMMUN-ELECTROPHORESIS IN AGAR GEL
- INTRODUCTION
- APPARATUS
- PRACTICAL DETAILS OF TECHNIQUE
- REFERENCES
- CHAPTER 8. OPTICAL ROTATION
- 1. HISTORICAL INTRODUCTION
- 2. PRINCIPLE
- 3. APPARATUS
- 4. METHOD
- CHAPTER 9. INFRA-RED SPECTRA
- INTRODUCTION
- THE TERMINOLOGY OF ABSORPTION SPECTROSCOPY
- THE NATURE OF INFRA-RED SPECTRA
- APPARATUS
- PREPARATION OF SPECIMENS
- EXPERIMENTAL PROCEDURE
- THE INTERPRETATION OF DICHROISM IN TERMS OF MOLECULAR STRUCTURE AND ORIENTATION
- THE VIBRATIONAL MODES OF PROTEINS AND POLYPEPTIDES
- APPLICATIONS OF THE METHOD
- REFERENCES
- CHAPTER 10. ANALYTICAL INFORMATION FROM TITRATION CURVES
- 1. INTRODUCTION
- 2. THE NATURE OF THE ACID- AND BASE-BINDING CAPACITY OF PROTEINS
- 3. THE PRINCIPLES OF TITRATION CURVE DETERMINATION
- 4. PRACTICAL DETERMINATION OF TITRATION CURVES
- 5. RESULTS
- 6. INTERPRETATION OF TITRATION CURYES
- 7. TITRATIONS IN MODIFIED SOLVENTS
- 8. TITRATION CURVES OF MODIFIED PROTEINS
- 9. SUMMARY OF INFORMATION AVAILABLE FROM TITRATION CURVES
- APPENDIX I
- REFERENCES
- CHAPER 11. X-RAY DIFFRACTION
- 1. HISTORICAL INTRODUCTION
- 2. FUNDAMENTAL THEORY
- 3. APPARATUS AND TECHNIQUES FOR EXPOSURE
- 4. THE APPLICATION OF X-RAY METHODS IN BIOCHEMISTRY
- 5. SOME X-RAY DATA ON CRYSTALLIZED PROTEINS, AMINO ACIDS, PEPTIDES, AND DINITROPHENYL AMINO ACIDS
- 6. CRITICAL NOTES
- REFERENCES
- CHAPTER 12. METHODS OF SEQUENCE ANALYSIS IN PROTEINS
- INTRODUCTION
- INSULIN
- RIBONUCLEASE
- HUMAN HAEMOGLOBIN
- ß-MELANOCYTE-STIMULATING HORMONE (ß-MSH)
- AUTHOR INDEX
- SUBJECT INDEX
