Manual of Sperm Function Testing in Human Assisted Reproduction
Published on 31. March 2021
978-1-108-88189-0 (ISBN)
Description
Selecting good-quality sperm for use in in-vitro fertilization is a key step in assisted reproduction. For many years purely morphological attributes have been used to assess suitability, but increasingly biochemical and molecular biological techniques are now identifying sperm with the best chances of producing viable and healthy embryos. Focusing on modern sperm function testing, this manual provides technical details of commonly used tests and gives an overview of the laboratory techniques used to evaluate sperm samples. Covering a variety of testing methods in detail, from manual and computer-assisted semen analysis to zona pellucida binding assays, and tests assessing sperm DNA damage such as the TUNEL assay. Describing the underlying science, practical advice for performing the tests is given, including tips for optimizing outcomes and trouble-shooting. This is an essential guide for reproductive medicine specialists, clinical andrologists, urologists and gynecologists working with sub-fertile men.
Reviews / Votes
'This is truly a compendium of human sperm function assays. The chapters include background information, some detailed protocols, interesting and insightful drawings, and photographs and insights from the authors/creators of the assays ... it should have a spot on the shelves of every biologist with an interest in human sperm function.' Karla Saner Amigh, Doody's NotesMore details
Language
English
Place of publication
Cambridge
United Kingdom
Target group
Professional and scholarly
Illustrations
Worked examples or Exercises
File size
12,61 MB
ISBN-13
978-1-108-88189-0 (9781108881890)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
Additional editions
Ashok Agarwal | Ralf Henkel | Ahmad Majzoub
Manual of Sperm Function Testing in Human Assisted Reproduction
Book
04/2021
Cambridge University Press
€58.00
Shipment within 15-20 days
Persons
Content
Introduction R. John Aitken; 1. Standard semen analysis: manual semen analysis Lars Bjoerndahl; 2. Standard semen analysis: computer-assisted semen analysis Liana Maree; 3. Standard semen analysis: home sperm testing Manesh Panner Selvam Kumar, Christopher Douglas and Ashok Agarwal; 4. Standard semen analysis: leukocytospermia Renata Finelli and Ralf Henkel; 5. Standard semen analysis: morphology Roelof Menkveld; 6. Sperm vitality: eosin-nigrosin dye exclusion Rakesh Sharma and Ashok Agarwal; 7. Sperm vitality: hypo-osmotic swelling test Alexandre Rouen and Rajasingam S. Jeyendran; 8. Determination of mitochondrial membrane potential by flow cytometry in human sperm cells Nicolas Germain, Nathalie Jouy, Carole Marchetti and Philippe Marchetti; 9. Capacitation and acrosome reaction: fluorescence techniques to determine acrosome reaction Raul S. Sanchez Gutierrez, Fabiola Zambrano and Pamela Uribe; 10. Capacitation and acrosome reaction: histochemical techniques to determine acrosome reaction Brett Nixon, Shenae Cafe, Elizabeth Bromfield, Geoffry De Iuliis and Matthew Dun; 11. Zona binding: competitive sperm-binding assay De Yi Liu, Ying Zhong and Zi-Na Wen; 12. Zona binding: hemizona assay Shubhadeep Roychoudhury and Daniel Franken; 13. Oolemma binding: sperm penetration assay Anup Shah and Kathleen Hwang; 14. Oxidative stress testing: direct tests Renata Finelli, Manesh Kumar, Panner Selvam and Ashok Agarwal; 15. Oxidative stress testing: indirect tests Rakesh Sharma, Kathy Robert and Ashok Agarwal; 16. Chromatin condensation: aniline blue stain Jesse JP, Vidhu Dhawan and Rima Dada; 17. Chromatin condensation: chromomycin A3 (CMA3) stain Sara Marchiani, Lara Tamburrino, Francesca Benini, Sandra Pellegrini and Elisabetta Baldi; 18. Sperm chromatin structure: Toluidine Blue Staining Mohammed Nasr-Esfahani and Marziyeh Tavalaee; 19. DNA damage: TdT-mediated dUTP nick-end-labelling (TUNEL) assay Rakesh Sharma, Concetta Iovine and Ashok Agarwal; 20. DNA damage: sperm chromatin structure assay (SCSA) Don Evenson; 21. DNA damage: COMET assay Luke Simon, Benjamin R. Emery and Douglas T. Carrell; 22. DNA damage: halo sperm test Jaime Gosalvez and Jose Luis Fernandez; 23. DNA damage: fluorescent in-situ hybridization (FISH) Sezgin Guenes; 24. Clinical value of sperm function tests Ahmad Majzoub; 25. Future developments: sperm proteomics Manesh Panner Selvam Kumar, Saradha Baskaran and Ashok Agarwal; 26. Conclusion Ahmad Majzoub, Ralf Henkel and Ashok Agarwal.
