Advanced Methodologies to Study and Enhance Cellular Cytotoxicity Against Cancer

1. An ex vivo assay to assess CAR T cell cytotoxicity in viable tumor tissues using a two-step staining protocolEmmanuel intégrative des tumeurs et immunothérapie Donnadieu, Irena Rajnpreht and Lene Vimeux2. new protocol to measure mitochondria polarization/function at the immunological synapseManuel Izquierdo, Javier Ruiz Navarro and Victor Calvo3. Analysis of CAR-T cell-mediated cytotoxicity in solid tumor modelsTeresa Lozano, Noa Beatriz Martín-Cófreces, Álvaro Gómez-Morón and Celia Martín-Otal4. Investigation of the in vitro lytic capacity of CAR-T by microscopy'Noa Beatriz Martín-Cófreces, Teresa Lozano, Álvaro Gómez-Morón and Celia Martín-Otal5. Antigen-specific T cell cytotoxicity toward target tumor cellsChong Sun and Helena Engel6. In vitro expansion of stem-like memory T cells via D-mannose supplementationBo Huang, Guideng Li, Yajing Qiu and Cngcong Zhao7. High-throughput time-lapse imaging of B16 target cell killing by ovalbumin-specific CD8+ T cells.Hélène Sophie DANIELS-TREFFANDIER, Loic Dupre, Beatriz CHAVES and Nicolas SOCQUET-JUGLARD8. Evaluation of an intracellular magneto-mechanical approach for regulating dendritic cell activationYu Cheng, Rui Gao, Wanxin Huang and Yehe Kang9. Analyzing the effect of NK cells on in-vivo models of tumor dissemination by digital pathology and flow cytometryMassimo Vitale, Paola Orecchia, Guido Ferlazzo, Monica Parodi, Simonetta Astigiano, Valentina Delli Zotti, Filippo Piombo, Stefania Martini, Simona Pigozzi and Paolo Carrega10. Super-resolution imaging of the NK cell synapse on supported lipid bilayers using MINFLUX nanoscopy.Charles Daniel Murin, Emily Mace, Patrick Ross, Jessica Matthias, Daniel Leaman, Micahel Zwick and Scott Henderson11. Assessment of NK cell cytotoxicity induced by il-15 based immunotherapy against cancer cellsCarole Seguin-Devaux, Camille Rolin, Gilles Iserentant and Aubin Pitiot12. Combined phenotyping and functional analysis of isolated human natural killer cells using spectral flow cytometrySophie-Christin Linkenbach, Ayline Kübler and Tommaso Torcellan13. Anti-tumor activity characterization of NK cell subsets by flow cytometryMassimo Vitale, Carola Perrone, Paolo Canevali, Veronica Colamassaro, Elena Giusto, Silvia Tamburini, Gianluca Ubezio, Guido Ferlazzo and Raffaella Meazza14. DNA Origami-Decorated Lipid Bilayer Systems for Interrogating the Spatial Requirements underlying B cell Immune-Synapse FormationBebhinn Treanor, Yuchen Hou, Chung -Yi Tseng, Raysa Orin, Emily Cohen and Leo Chou