2D and 3D Cellular Screening Models and AI Guided Analysis
Elsevier (Publisher)
Published on 1. April 2026
318 pages
978-0-443-42852-4 (ISBN)
Description
2D and 3D Cellular Screening Models and AI Guided Analysis, Volume 204 explores the development, application, and comparative benefits of 2D monolayer cultures and 3D organoids, spheroids, and tissue-engineered constructs for biomedical research and drug discovery. Chapters in this new release include High throughput CellTiter-glo viability assessment, Assessing viability of cancer cell spheroids in 3D drug screening, 3D killing assay of cancer spheroids by cytotoxic T lymphocytes in anchored microfluidic droplets, Use of micropatterning for 3D cell culture, Use of microfluidic chip devices for assessing the effect of radiation on 3D cell culture models, and much more.Additional chapters cover A 3D model for assessing regulated cell death modalities, YOLO detection model for Cell Viability assessment, Bioinformatics and AI/ML approaches using multi-omics data to accelerate diagnosis and delivery of precision care, Automated image analysis and machine learning for the assessment of mitochondrial membrane potential, Assessing cancer cell heterogeneity in patient-derived tumoroids, Assessment of lysosomal drug sequestration and release using fluorescence microscopy, Assessment of T cell toxicity in ex-vivo models of cancer, Three-dimensional Organoid Screening using Leica Thunder Microscope, Using vascularized organoids-on-chip for assessing systemic drug delivery, and many more interesting and timely topics.
- Includes detailed methodologies and protocols for developing both 2D monolayer and 3D spheroid/organoid cellular screening models
- Presents in-depth comparisons of the advantages, limitations, and applications of 2D vs. 3D cellular models in research and drug discovery
- Provides high-throughput and high-content screening methods for assessing cellular responses
More details
Other editions
Additional editions
Oliver Kepp | Guido Kroemer
2D and 3D Cellular Screening Models and AI Guided Analysis
Book
04/2026
Academic Press
€172.08
Shipment within 15-20 days
Persons
Content
1. PrefaceGuido Kroemer and Oliver Kepp2. High throughput CellTiter-glo viability assessmentDana Alawieh and Aline Renneville3. Assessing viability of cancer cell spheroids in 3D drug screeningOliver Kepp and Marion Leduc4. 3D killing assay of cancer spheroids by cytotoxic T lymphocytes in anchored microfluidic dropletsCharles Baroud5. Use of micropatterning for 3D cell cultureEmna Ouni and Kristine Schauer6. Use of microfluidic chip devices for assessing the effect of radiation on 3D cell culture modelsPhilip Wong, Rodin Chermat, Elena Refet-Mollof, Maryam Ziaee, Julie Lafontaine and Thomas Gervais7. A 3D model for assessing regulated cell death modalitiesDmitri Krysko and Robin Demuynck8. YOLO detection model for Cell Viability assessmentFelix Peyre9. Bioinformatics and AI/ML approaches using multi-omics data to accelerate diagnosis and delivery of precision careZeeshan Ahmed, Kritika Singh, Sarah Usman, Saman Zeeshan, Naveena Yanamala, Vikram Bhise, Mark Nichols, Sabahat Bokhari and Partho Sengupta10. Automated image analysis and machine learning for the assessment of mitochondrial membrane potentialMarta Giacomello, Camilla Bean, Federica Dal bello, Emad Norouzi Esfahani, Alessio Gianelle, Saradha Ramesh, Luca Persano, Elena Rampazzo and Caterina Vianello11. Assessing cancer cell heterogeneity in patient-derived tumoroidsFlora Doffe12. Assessment of lysosomal drug sequestration and release using fluorescence microscopyKarla Alvarez, Mojgan Djavaheri-Mergny, Guido Kroemer, Lucille Ferret, Samy Dehissi and Jean Guillon13. Assessment of T cell toxicity in ex-vivo models of cancerEmmanuel intégrative des tumeurs et immunothérapie Donnadieu and Alice Machado14. Three-dimensional Organoid Screening using Leica Thunder MicroscopeManoliu Tudor15. Using vascularized organoids-on-chip for assessing systemic drug deliveryXavier Gidrol, Emily Tubbs, Fabrice P Navarro and Yves Fouillet16. Quantitative assessment of nanoparticle uptake and trafficking in 3D cell culture modelsMeritxell B. Cutrona17. Fluorescence microscopic quantitation of lysosomal cholesterol accumulation induced by cationic amphiphilic drugsMojgan Djavaheri-Mergny, Karla Alvarez, Samy Dehissi, Lucille Ferret and Guido Kroemer
