Yeast Protocols
Description
This fourth edition provides all new methods on yeast that have not been included in the past three editions. Divided in four parts, chapters guide readers through general applications for genetic manipulation in yeast cells, genome-wide studies, systematically rearrange the yeast chromosomes, and pathway methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Yeast Protocol, Fourth Edition aims to compliment previous editions while offering new tools for the yeast research community.
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Content
1. Use of Yeast Plasmids - Transformation and Inheritance Assays
Anastasiia Mereshchuk, Joyce S.K. Chew, and Melanie J. Dobson
2. Construction of Tight Conditional Mutants Using the Improved Auxin-inducible Degron (iAID) Method in the Budding Yeast Saccharomyces cerevisiae
Seiji Tanaka
3. CRISPR Nickase-Mediated Base Editing in Yeast
Kouichi Kuroda and Mitsuyoshi Ueda
4. Genomic Promoter Shuffling by Using Recyclable Cassettes
Xuelei Tian, Wenqing Zhang, and Wei Xiao
5. Study Essential Gene Functions by Plasmid Shuffling
Li Fan and Wei Xiao
6. Scarless Genomic Protein Labeling in Saccharomyces cerevisiaeQian Wang, Yu V. Fu, and Wei Xiao
7. High-Copy Yeast Library Construction and High-Copy Rescue Genetic Screen in Saccharomyces cerevisiae
Fanli Zeng and David G. Quintana
8. Genome-Wide Imaging-Based Phenomic Screening Using Yeast (Saccharomyces cerevisiae) Strain Collections
Xiuling Cao, Xuejiao Jin, and Beidong Liu
9. Applications of Oxford Nanopore Sequencing in Schizosaccharomyces pombe
Ming He, Xu Chi, and Jie Ren
10. Measuring Genome-Wide Nascent Nucleosome Assembly Using ReIN-Map
Zhiyun Xu, Jianxun Feng, and Qing Li
11. Targeted Integration of Complex Genetic Elements at Multi-Copy Loci by Golden Gate Assembly
Yixuan Zhu and Limin Cao
12. Chromosomal Rearrangements of Synthetic Yeast by SCRaMbLE
Zhouqing Luo, Shuangying Jiang, and Junbiao Dai
13. Use YeastFab to Construct Genetic Parts and Multicomponent Pathways for Metabolic Engineering
Shuangying Jiang, Zhouqing Luo, and Junbiao Dai
14. A Versatile Protocol to Generate Translocations in Yeast Genomes using CRISPR/Cas9
Nicolas Agier, Aubin Fleiss, Stéphane Delmas, and Gilles Fischer
15. Yeast Nucleoplasmic Extracts and an Application to Visualize Chromatin Assembly on Single Molecules of DNA
Yong Wang and Yu V. Fu
16. Assays for Autophagy III: Observing Dynamic Protein Trafficking
Jing Zhu and Zhiping Xie
17. Isolation of Aged Yeast Cells Using Biotin-Streptavidin Affinity Purification
Xuejiao Jin, Xiuling Cao, and Beidong Liu
18. High-Throughput Rapid Yeast Chronological Lifespan Assay
Abd-Al-Wahab A. Khawaja, Zach R. Belak, Christopher H. Eskiw, and Troy A. A. Harkness
19. Long-Term Imaging and Dynamic Analysis of Cytoophidia in Yeast
Shanshan Zhang, Hui Li, and Ji-Long Liu
20. Monitoring 5'-end Resection at Site-specific Double-strand Breaks by Southern Blot Analysis
Haoyang Peng, Simin Zhang, and Xuefeng Chen