Neuroscience Protocols: Module 6

Section 010 - Behavioral Neurobiology. A lever-release version of the conditioned avoidance response (CAR) paradigm for assessing the neuronal mechanisms of action of antipsychotic drugs (I.M. White, G.V. Rebec). A protocol for schedule control of intracranial self-stimulation (G.J. Schaefer). Section 020 - Cellular and Molecular Neurobiology. Construction of mammalian brain size-fractionated cDNA expression library and expression cloning of neuropeptide receptor by ligand binding (G.-x. Xie, K. Maruyama, A. Miyajima). Differential display of gene expression in rat focal brain ischemia (X. Wang, T.-L. Yue, F.C. Barone, R.R. Ruffolo, G.Z. Feuerstein). A sensitive quantitative RNase protection assay for identifying polyadenylation sites on a neuropeptide messenger RNA (C.J. Keiger, G. Brewer, M. Sorci-Thomas, J.C. Rose). Section 030 - Developmental Neurobiology. Retrograde and anterograde axonal tracing with fluorescent dextran-amines in the embryonic nervous system (J.C. Glover). Induction of arhinencephaly using fetal laser surgery exo utero in mice (I. Naruse, A.G. Sato, H. Keino, M. Taniguchi). Investigation and developmental changes in protein substrates of Ca 2+ /calmodulin-dependent protein kinase II in the rat forebrain (T. Yamauchi, H. Sugiura, T. Hirabayashi). Section 040 - Neuroimmunology. Neonatal tolerization and rapid immunization: an immunological subtraction technique for the generation of monoclonal antibodies (P.C. Kind, S. Hockfield). Section 050 - Neuromorphology. Staining myelin and myelin-like degradation products: glycol methacrylate resin-embedding combined with Sudan black B staining (P.O. Gerrits, G. Holstege). Silver staining of traumatized neurons: application of a Gallyas procedure in experimental cerebral hypoxia/ischemia research (G.J. Ter Horst, S. Knollema, M.F. Knigge, H.J. Krugers, S.V. Van de Witte, F. Postema, H.W. Hom). Mapping of the human brain in normal and pathological situations: the single cell and fiber level, employing Lucifer yellow microinjection, carbocyanine dye tracing, immunofluorescence, and 3D confocal laser scanning microscopy reconstruction (P.V. Belichenko, A. Dahlstrom). NADPH-diaphorase staining in the central nervous system (J.R. Alonso, R. Arevalo, A. Porteros, J.G. Bri#on, E. Garcia-Ojeda, J. Aijon). Triple staining for parvalbumin, calretinin and carbonic anhydrase in spinal primary afferent neurons: combination of double immunohistochemistry and enzyme histochemistry (H. Ichikawa, T. Deguchi, D.M. Jacobowitz, T. Sugimoto). Section 060 - Neuropharmacology and Neurochemistry. Intracerebral dialysis in freely moving rats for the study of changes in monoamine metabolism in the nucleus accumbens correlated with locomotor activity (R. Sugita, K. Terada, Y. Sekiya, Y. Sawa, S. Nomura, T. Nakazawa). Section 070 - Neurophysiology. Estimation of electroencephalographic potential flow (T. Inouye, K. Shinosaki, S. Toi, Y. Matsumoto, N. Hosaka).