From Genes to Clones
Introduction to Gene Technology
Published on 7. June 1987
Book
Paperback/Softback
XIII, 634 pages
978-3-527-26644-9 (ISBN)
Description
This textbook on gene technology offers for the first time a unified approach to the subject and summarizes the concepts and strategies behind the art of gene cloning. It is the only book available that covers all technical matters. The author, a well-known teacher and scientist, describes the unit operations of gene technology and provides practical guidance on the isolation and characterization of DNA and genes, the development of cloning vectors, and the characterization of recombinant DNA molecules. He also discusses in great detail the development and practical applications of plasmids, bacteriophages, cosmids, phasmids, and eukaryotic viruses as cloning vehicles, along with the respective hosts, i.e., prokaryotes, streptomyces, yeasts, plant cells, and eukaryotes. The book, a completely revised and updated translation of the best-selling German text, leads to the frontiers of current knowledge. Each chapter contains an extensive list of references with the most recent literature up to 1986.
The appendix includes information about frequently used host straints, restriction enzymes, restriction maps, sequence data, and the 1986 NIH Guidelines for Research Involving Recombinant DNA Molecules. A very detailed index and more than 400 figures allow the book to be used as a reference manual.
The appendix includes information about frequently used host straints, restriction enzymes, restriction maps, sequence data, and the 1986 NIH Guidelines for Research Involving Recombinant DNA Molecules. A very detailed index and more than 400 figures allow the book to be used as a reference manual.
More details
Series
Language
English
German
Place of publication
Weinheim
Germany
Target group
College/higher education
Professional and scholarly
Illustrations
438 Abb., 54 Tab.
Dimensions
Height: 250 mm
Width: 170 mm
Weight
1250 gr
ISBN-13
978-3-527-26644-9 (9783527266449)
Schweitzer Classification
Persons
Content
Isolation, Identification, and Characterisation of DNA Fragments; Restriction Endonucleases; Preparation and Cloning of Eukaryotic cDNA; Chemical Synthesis of DNA; DNA Sequencing; Ligation of DNA Fragments; Ligation with DNA Ligases; 'In vivo' Ligation; 'E. coli' Vectors; Plasmids; Bacteriophage as Cloning Vehicle; Cloning in Yeasts; The Life Cycle of 'Saccharomyces cerevisiae'; Genetics of 'Saccharomyces cervisiae'; Identification of Yeast Genes; Genetic Markers and Selection Systems; Vectors; Gene Expression in Eukaryotes; Yeast Expression Vectors; Cloning in Gram-positive Bacteria; Cloning in 'B. subtilis'; Cloning in Streptomycetes; Expression Vectors in Prokaryotes; 'E. coli' Promoters Ribosome Binding Sites; Codon Selection; Construction of Expression Vectors; Maturation, Transport, and Stability of Eukaryotic Proteins in Bacteria; Conclusions; Eukaryotic Vectors; Selectable Marker for Eukaryotic Cells; Simian Virus; SV40 Vectors; Stimulating and Inhibiting DNA Sequences; Methods for Transfection of Viral DNA; Papilloma Viruses as Vectors; Retroviruses as Vectors; Gene Expression by Gene Amplification; Gene Transfer into Drosophila; Vaccinia Virus Vectors; Genomic Libraries; Isolation of Genomic DNA Fragments; Vectors; Ligation Techniques; Selection Techniques; Cloning in Plants; Fusion of Somatic Cells; DNA Transfer; Identification of Recombinant DNA; Direct Methods; Indirect Methods; Directed Mutagenesis; Deletions; Insertions; Localised Point Mutations; Directed Mutagenesis by Synthetic Oligonucleotides; Safety in Recombinant DNA Research; Laboratory and Industrial Applications; Environmental Applications; Reproductive Engineering.