In Situ Hybridization
D. G. Wilkinson(Author)
Oxford University Press
Published on 1. October 1992
Book
Hardback
180 pages
978-0-19-963328-9 (ISBN)
Article exhausted; check for reprint
Description
The detection of specific nucleic acid sequences by in situ hybridization has many important applications in modern biology. In situ detection of viral DNA sequences is enabling the diagnosis of human diseases, the detection of specific sequences on chromosomes is crucial for genome analysis, and in fields such as developmental biology and neurobiology, the investigation of gene expression by in situ hybridization to mRNA has become a key technique. "In Situ Hybridization - A Practical Approach" is a comprehensive practical guide to the uses of this major technique. Step-by-step protocols are presented for the preparation of cloned DNA, RNA and oligonucleotide probes, with both radioactive and non-radioactive labels, and their hybridization to mRNA, viral DNA, and chromosomal DNA. Methods are provided for the analysis of cells, tissue sections, and whole embryos. In addition, techniques are presented for carrying out in situ hybridization in combination with the immunochemical detection of protein, and for using it at the electron microscopic level. This book is aimed at any researcher wishing to exploit the many widesprad applications of this important technique.
More details
Language
English
Place of publication
Oxford
United Kingdom
Target group
College/higher education
Professional and scholarly
Illustrations
5 line drawings, 11 half-tones, 5 colour illustrations, bibliography, index
ISBN-13
978-0-19-963328-9 (9780199633289)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Classification
Other editions
New editions
D. G. Wilkinson
In Situ Hybridization
Book
02/1999
2nd Edition
Oxford University Press
€80.60
Article exhausted; check different version
Content
The theory and practice of in situ hybridization, D.G. Wilkinson; in situ hybridization to cellular RNA with radiolabelled RNA probes, L.M. Angerer and R.C. Angerer; in situ hybridization with oligodeoxyribonucleotide probes, W. Scott Young III; in situ hybridization with biotinylated probes, P. Emson and M.J. Gait; whole mount in situ hybridization in Drosophila, D. Tautz et al; whole mount in situ hybridization of vertebrate embryos, D.G. Wilkinson; simultaneous detection of cellular RNA and proteins, M. Brahic and S. Ozden; in situ hybridization at the electron microscope level, M. Binder; detection of virus in infected human tissue by in situ hybridization, F.A. Lewis and M. Wells; in situ hybridization to chromosomes with biotinylated probes, E. Viegas-Pequignot.