Platelets
Published on 1. March 1996
Book
Hardback
396 pages
978-0-19-963538-2 (ISBN)
Description
Platelets are small blood cells that play a vital role in blood clotting. They are essential for wound healing, but their unwanted action can lead to ailments such as stroke and heart attack. This volume describes state-of-the-art techniques for the experienced researcher, both in academia and industry. Methodology is also included that will enable the newcomer to perform routine experiments. Techniques covered range from basic procedures such as platelet preparation to the latest methodology, such as monitoring calcium levels in single cells, protein tyrosine phosphorylation and PCR.
More details
Language
English
Place of publication
Oxford
United Kingdom
Target group
College/higher education
Professional and scholarly
Illustrations
halftones, line figures, tables, bibliography
ISBN-13
978-0-19-963538-2 (9780199635382)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Classification
Persons
Content
Preface. Acknowledgement. 1: Platelet preparation and functional responses. 2: Cellular model systems to study megakaryocyte differentiation. 3: Techniques in the preparation of semi-permeable platelets and their uses. 4: Use of fluorescent indicators to measure intracellular Ca2+ and other ions. 5: Preparation of highly purified human platelet plasma and intracellular membranes using high voltage free flow electrophoresis and methods to study Ca2+ regulation. 6: The use of flow-cytometry to study platelet activation. 7: Identification, isolation, and characterization of platelet glycoproteins mediating platelet adhesion. 8: Monoclonal antibodies to platelet cell surface antigens and their use. 9: Measurement of protein phosphorylation, kinase activity, and G protein function in intact platelets and membrane preparations. 10: Tyrosine phosphorylation. 11: Study of proteins associated with the platelet cytoskeleton. 12: Methods for measuring agonist-induced phosphlipid metabolism in intact human platelets. 13: Cyclic nucleotides: measurement and function. 14: Immunocytochemical and electron microscope studies of platelets. 15: Clinical aspects of platelet function. 16: Platelet procoagulent activity and its measurement. 17: The study of platelet function in other species. List of suppliers. Index