The Protein Protocols Handbook

Name: The Protein Protocols Handbook
Brand: Humana Press Inc.
Price: 85.59 EUR
Availability: OutOfStock

John M. Walker(Editor)

Humana Press Inc.

2nd Edition

Published on 19. February 2002

Book

Paperback/Softback

XXIV, 1146 pages

978-0-89603-941-4 (ISBN)

€85.59incl. 7% vat

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Description

The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular BiologyT series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully, the Methods section contains detailed st- by-step descriptions of every protocol that should result in the successful execution of each method. The Notes section complements the Methods material by indicating how best to deal with any problem or difficulty that may arise when using a given technique, and how to go about making the widest variety of modifications or alterations to the protocol. Since the first edition of this book was published in 1996 there have, of course, been significant developments in the field of protein chemistry.

Reviews / Votes

"the breadth of techniques covered is large and this alone makes the book of practical value...The protocols are provided with sufficient detail so as to allow implementation as new methods in any working laboratory...It can easily be envisioned that this book would become the subject of a tug of war in a busy laboratory. Most investigators will find value here as will students working in almost any area dealing with proteins...This is a compilation not found in other single sources. The very broad scope has considerable practical value. I personally want this in my laboratory."-Doody's Health Sciences Book Review Journal "The wide acceptance of this handbook in the former edition has resulted to this second thoroughly revised and extended second edition considering the recent significant developments in the field of protein chemistry. It is an excellent collection of methods and protocols, giving an essential and compact guide for those new as well as those well established to protein laboratory techniques." - Ernahrungforschung Review of the first edition: "The Protein Protocols Handbook has arrived at the right place at the right time."-Trends in Cell Biology From Reviews of the First Edition "...a must for researchers in protein chemistry and especially those with less experience..." -Doody's Health Sciences Book Review Journal "...a very useful tool for daily work.." -Nahrung-Food "...definitely an indispensable benchtop handbook...highly recommended for all levels of research within the field." -Bioseparation "...stands out in an already overcrowded field..." -Aslib Book Guide "In his preface the editor wishes the reader luck in his protein analysis, his book represents an invaluable compendium to help achieve this aim." - Molecular Biotechnology

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Content

Part I. Quantitation of Proteins
1 Protein Determination by UV Absorption
Alastair Aitken and Michèle P. Learmonth
2 The Lowry Method for Protein Quantitation
Jakob H. Waterborg
3 The Bicinchoninic Acid (BCA) Assay for Protein Quantitation
John M. Walker
4 The Bradford Method for Protein Quantitation
Nicholas J. Kruger
5 Ultrafast Protein Determinations Using Microwave Enhancement
Robert E. Akins and Rocky S. Tuan
6 The Nitric Acid Method for Protein Estimation in Biological Samples
Scott A. Boerner, Yean Kit Lee, Scott H. Kaufmann, and Keith C. Bible
7 Quantitation of Tryptophan in Proteins
Alastair Aitken and Michèle P. Learmonth
8 Flow Cytometric Quantitation of Cellular Proteins
Thomas D. Friedrich, F. Andrew Ray, Judith A. Laffin, and John M. Lehman
9 Kinetic Silver Staining of Proteins
Douglas D. Root and Kuan Wang

Part II. Electrophoresis of Proteins and Peptides and Detection in Gels
10 Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins
John M. Walker
11 SDS Polyacrylamide Gel Electrophoresis of Proteins
John M. Walker
12 Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins
John M. Walker
13 SDS-Polyacrylamide Gel Electrophoresis of Peptides
Ralph C. Judd
14 Identification of Nucleic Acid Binding Proteins Using Nondenaturing Sodium Decyl Sulfate Polyacrylamide Gel Electrophoresis (SDecS-Page)
Robert E. Akins and Rocky S. Tuan
15 Cetyltrimethylammonium Bromide Discontinuous Gel Electrophoresis of Proteins: Mr-Based Separation of Proteins with Retained Native Activity
Robert E. Akins and Rocky S. Tuan
16 Acetic-Acid-Urea Polyacrylamide Gel Electrophoresis of Basic Proteins
Jakob H. Waterborg
17 Acid-Urea-Triton Polyacrylamide Gel Electrophoresis of Histones
Jakob H. Waterborg
18 Isoelectric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels
John M.Walker
19 Protein Solubility in Two-Dimensional Electrophoresis: Basic Principles and Issues
Thierry Rabilloud
20 Preparation of Protein Samples from Mouse and Human Tissues for 2-D Electrophoresis
Joachim Klose
21 Radiolabeling of Eukaryotic Cells and Subsequent Preparation for 2-D Electrophoresis
Nick Bizios
22 Two-Dimensional Polyacrylamide Gel Electrophoresis Using Carrier Ampholyte pH Gradients in the First Dimension
Patricia Gravel
23 Casting Immobilized pH Gradients (IPGs)
Elisabetta Gianazza
24 Nonequilibrium pH Gel Electrophoresis (NEPHGE)
Mary F. Lopez
25 Difference Gel Electrophoresis
Mustafa Ünlü and Jonathan Minden
26 Comparing 2-D Electrophoretic Gels Across Internet Databases
Peter F. Lemkin and Gregory C. Thornwall
27 Immunoblotting of 2-D Electrophoresis Separated Proteins
Barbara Magi, Luca Bini, Sabrina Liberatori, Roberto Raggiaschi, and Vitaliano Pallini
28 Quantification of Radiolabeled Proteins in Polyacrylamide Gels
Wayne R. Springer
29 Quantification of Proteins on Polyacrylamide Gels
Bryan John Smith
30 Rapid and Sensitive Staining of Unfixed Proteins in Polyacrylamide Gels with Nile Red
Joan-Ramon Daban, Salvador Bartolomé, Antonio Bermúdez, and F. Javier Alba
31 Zinc-Reverse Staining Technique
Carlos Fernandez-Patron
32 Protein Staining with Calconcarboxylic Acid in Polyacrylamide Gels
Jung-Kap Choi, Hee-Youn Hong, and Gyurng-Soo Yoo
33 Detection of Proteins in Polyacrylamide Gels by Silver Staining
Michael J. Dunn
34 Protein Detection on Polyacrylamide Gels and Electroblots Using Transition Metal Chelate Stains
Wayne F. Patton
35 Detection of Proteins in Polyacrylamide Gels by Fluorescent Staining
Michael J. Dunn
36 Detection of Proteins and Sialoglycoproteins in Polyacrylamide Gels Using Eosin Y Stain
Fan Lin and Gary E. Wise
37 Electroelution of

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The Protein Protocols Handbook

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