Fibrosis Research
Methods and Protocols
Published on 8. August 2005
Book
Hardback
XVI, 392 pages
978-1-58829-479-1 (ISBN)
Description
Leading investigators review the highlights of current fibrosis research and the experimental methodologies used uncover the mechanisms that drive it. In their discussion of research methodologies utilizing cultured cells to model various aspects of the fibrotic response in vitro, the authors describe the isolation, characterization, and propagation of mesenchymal cells, and highlight the similarities and differences between methods that are appropriate for different types of fibroblasts. Approaches for studying collagen gene regulation and TGF-b production are also discussed, along with experimental methodologies utilizing animal models to study the pathogenesis of fibrosis. The protocols follow the successful Methods in Molecular MedicineT series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Reviews / Votes
"...a must for the shelf of every researcher and physician interested in fibrotic diseases." -RheumatologyMore details
Series
Edition
2005 ed.
Language
English
Place of publication
Totowa
United States
Target group
Professional and scholarly
Research
Illustrations
80 s/w Abbildungen
XVI, 392 p. 80 illus.
Dimensions
Height: 241 mm
Width: 160 mm
Thickness: 30 mm
Weight
857 gr
ISBN-13
978-1-58829-479-1 (9781588294791)
DOI
10.1385/1592599400
Schweitzer Classification
Other editions
Additional editions
Book
11/2010
Humana Press Inc.
€117.69
Shipment within 15-20 days
Content
The Clinical Scope of Fibrosis.- Pulmonary Fibrosis.- Renal Fibrosis.- Transforming Growth Factor-?.- Regulation of Extracellular Matrix Metabolism: Cultured Cells.- Isolation and Culture of Skin Fibroblasts.- Isolation and Culture of Hepatic Stellate Cells.- Isolation and Phenotypic Characterization of Lung Fibroblasts.- Methods for Measuring Type I Collagen Synthesis In Vitro.- Methods for Assessing the Molecular Mechanisms Controlling Gene Regulation.- Methods for Measuring TGF-? Using Antibodies, Cells, and Mice.- Studying Fibrosis Using Animal Models.- Morphological Methods for Assessment of Fibrosis.- Methods for Measuring Hydroxyproline and Estimating In Vivo Rates of Collagen Synthesis and Degradation.- Approaches to Evaluation of Fibrogenic Pathways in Surgical Lung Biopsy Specimens.- Animal Models for Adult Dermal Wound Healing.- Modeling Liver Fibrosis in Rodents.- Animal Models of Pulmonary Fibrosis.- Animal Models of Renal Fibrosis.- Animal Models of Cardiac Fibrosis.- Genetic Approaches to Studying Fibrosis.- Finding Fibrosis Genes.- Genetic Studies to Identify Hepatic Fibrosis Genes and SNPs in Human Populations.- Analysis of Microarray Experiments for Pulmonary Fibrosis.- DNA Microarrays and Data Mining To Study Hepatic Fibrosis.- Analysis of Proteins Dominantly Expressed in Hepatic Stellate Cells of Activated Phenotype.